Mutation Breeding of Neutral Phytase-Producing Strain Using Both UV Light and Lithium Chloride

Abstract

Abstract: Agrobacterium radiobacter AR01 was mutagenized using ultraviolet (UV) irradiation and subsequent lithium chloride treatment to obtain a high-yield neutral phytase-producing strain. The neutral phytase activity of the strain reached up to 18.49 U/mL after 96 h cultivation at pH 7.0, 30 ℃ and an inoculum amount of 10%, which was 47.68% higher than that of the original strain. The fermentation cycle was shortened by 12 h. Moreover, the mutant strain showed good hereditary stability.

Key words: neutral phytase, mutation induced by both UV and lithium chloride, enzymatic activity, stability

CLC Number:

S816.7
Q933

References

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