Optimization of Phosphorylation Process for Exopolysaccharide from Lactococcus lactis subsp. lactis

Abstract

Abstract: One-factor-at-a-time and orthogonal array design methods were employed to optimize the phosphorylation of exopolysaccharide from Lactococcus lactis subsp. lactis. The amount of grafted phosphate was investigated with respect to several process conditions such as the amount of added phosphorylate, reaction temperature, reaction time and pH. The optimal phosphorylation conditions were determined as 1:6 of exopolysaccharide-to-phosphorylate ratio, 90 ℃ of reaction temperature, 4 h of reaction time and pH 6.0. Under these conditions, the amount of grafted phosphate was up to 1.639 mg/g.

Key words: Lactococcus lactis subsp. lactis, exopolysaccharide, phosphorylation

CLC Number:

TS201.21

References

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