Orthogonal Array Optimization of Ultrasonic-Assisted Extraction of Panax notoginseng Saponin Rg1

Abstract

Abstract: The ultrasonic-assisted extraction of Panax notoginseng saponin Rg1 was optimized using an L9(34) orthogonal array design based on one-factor-at-a-time experiments. The effects of ethanol concentration, material-to-liquid ratio, ultrasonic frequency and extraction time on extraction efficiency were examined. Ethanol concentration had the highest effect on extraction efficiency, followed by extraction time and material-to-liquid ratio; the effect of ultrasonic frequency was the weakest. The optimal levels of ethanol concentraion, material-to-liquid ratio (g/mL), ultrasonic frequency and extraction time were 70%, 1:12, 59 kHz and 60 min, respectively. Under the optimized extraction conditions, the extraction yield of saponin from Radix Panax notoginseng was 1.85%.

Key words: Panax notoginseng, ultrasonic-assisted extraction, saponin Rg1

CLC Number:

TS205.7

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