Abstract:

Chlorophyllin was extracted from bamboo leaves and used to prepare oil-soluble copper chlorophyllin by
copper replacement and degreasing. The procedure of copper replacement critical for the quality of copper chlorophyllin
was optimized by response surface methodology based on a three-variable, three-level Box-Behnken design. The optimal
conditions for copper replacement were established as follows: the amount of copper was 2.2-fold of the theoretical
amount and the reaction was allowed to proceed for 75 min at 60 ℃. Under these conditions, the yield of copper
chlorophyllin was 4.051 mg/g. The main quality indicators of the final product obtained met the requirements for use as a
food additive.

Key words: bamboo leaves, chlorophyll, copper chlorophyllin, preparation, response surface methodology