Abstract:

Natural fermentation of soybean yellow serofluid for acid production was investigated. Fermentation
conditions including temperature, time, inoculum amount and glucose addition were optimized by uniform design
to obtain the optimum acid production and pH. Quadratic polynomial regression analysis showed that the optimum
fermentation conditions were 37 ℃, inoculum amount of 3%, 46 h, 2 g/100 mL glucose, initial pH 6.2. The maximum acid
production under these conditions was 0.7815 g/100 mL.

Key words: Suanjiang (fermented soybean yellow serofluid), natural fermentation, acid production, uniform design, optimization