Effect of Ganoderma atrum Polysaccharide on cAMP/PKA Pathway in Mouse Colon Cancer Cells

doi:10.7506/spkx1002-6630-201323055

Abstract

Abstract:

Ganoderma atrum polysaccharide-1 (PSG-1) has been reported to be natural chemopreventive in several types
of tumor cells. In our previous study, we found that PSG-1 could significantly inhibit the tumor growth. We hypothesized
that PSG-1 might exert its anticancer effect by activating cAMP/protein kinase A (PKA) pathway. The present study was
designed to analyze the effect of PSG-1 on the cAMP/PKA pathway in mouse colon cancer CT26 cells. The cells were
treated with 0, 50, 100 μg/mL, and 200 μg/mL PSG-1 or 20 μg/mL 5-fluorouracil, respectively, for 48 h. The ratio of
apoptotic CT26 cells was measured by flow cytometry analysis. The cellular cAMP content was tested using a cAMP assay
kit. The activity of PKA was measured using a PKA activity assay kit. Reverse transcript-polymerase chain reaction (RT-PCR)
was used to analyze the mRNA expressions of PKA and cAMP response element binding protein (CREB) of the cells. The
results showed that PSG-1 significantly induced the apoptosis of the CT26 cells and that cAMP concentration in the cells
treated with PSG-1 was dramatically increased by 20.18%, 38.67% and 56.25% (P ＜ 0.05), respectively. The PKA activity
was increased by 15.42%, 18.76% and 38.26%, respectively. The mRNA levels of both PKA and CREB of the cells treated
with PSG-1 were increased. The current study suggests that PSG-1 activates the cAMP/PKA pathway in colon cancer cells
by inducing cAMP production and activating PKA protein.

Key words: Ganoderma atrum polysaccharide, CT26 cells , cAMP/PKA pathway

CLC Number:

Q949.32

ZHANG Shen-shen，NIE Shao-ping，XIE Ming-yong*. Effect of Ganoderma atrum Polysaccharide on cAMP/PKA Pathway in Mouse Colon Cancer Cells[J]. FOOD SCIENCE, 2013, 34(23): 271-275.

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