Abstract: Objective: To investigate the protective effect of Ganoderma atrum polysaccharides (GAP) against oxidative injury induced by acrylamide (AA) in intestinal epithelial cells (IEC)-6. Methods: The MTT assay was used to evaluate the proliferation of IEC-6. After cultivation, the cells and culture medium were collected for determining the activities of lactate dehydrogenase (LDH), malondialdelyde (MDA), superoxide dismutase (SOD) and reduced glutathione peroxidase (GSHPx). Results: GAP had no toxic effect on the cultured IEC-6 at the tested concentrations. Compared with the AA-treated group, GAP significantly attenuated AA-induced oxidative damage and increased cell viability by decreasing LDH activity and MDA content, and increasing SOD and GSH-Px activities in IEC-6 line. Conclusion: These results suggested that GAP showed a protective effect against AA-induced oxidative damage in IEC-6 through inhibiting lipid peroxidation, and increasing antioxidant system activity.

Key words: Ganoderma atrum polysaccharides, acrylamide, IEC-6, oxidative damage