FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (17): 182-187.doi: 10.7506/spkx1002-6630-20190731-430

• Nutrition & Hygiene • Previous Articles     Next Articles

Effect of Crude Alkaloids from Mulberry Leaves on Improving D-Galactose-Induced Oxidative Protein Damage in Mice

YANG Zhongmin, SHEN Yihong, HUANG Xianzhi, WANG Zuwen, DING Xiaowen   

  1. (1. Chongqing Key Laboratory of Agricultural Products Processing, National Demonstration Center for Experimental Food Science and Technology Education, College of Food Science, Southwest University, Chongqing 400716, China; 2. Science and Technology Department, Southwest University, Chongqing 400716, China)
  • Online:2020-09-15 Published:2020-09-16

Abstract: Objective: To explore the effect of alkaloids from mulberry leaves on improving oxidative protein damage in mice and the underlying mechanism for the purpose of providing a theoretical basis for the development and utilization of alkaloids from mulberry leaves. Methods: A mouse model of oxidative damage was induced by D-galactose (D-Gal) and administered with different doses of mulberry leaf alkaloids. At the end of the 8th week, the contents of protein carbonyl (PCO), advanced oxidation protein products (AOPP) and 3-nitrotyrosine (3-NT), and the enzyme activity levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and NAD(P)H quinine oxidoreductase 1 (NQO1) in plasma were measured. The mRNA expression of SOD, GSH-Px, NQO1, nuclear erythroid related factor 2 (Nrf2) and Kelch-like ECH-associated protein-1 (Keap1) in liver tissues were also detected by real-time polymerase chain reaction (qPCR). Results: Compared with the model control group, the plasma PCO, AOPP and 3-NT levels of mice decreased by 36.43%, 61.81% and 58.13%, respectively after intragastric administration of high-dose mulberry leaf alkaloids (200 mg/kg mb) (P < 0.01). However, the activity of SOD, GSH-Px and NQO1 in plasma increased by 48.89%, 167.17% and 85.12% (P < 0.01). Meanwhile, high-dose mulberry leaf alkaloids also increased the mRNA expression of SOD1, SOD2, GSH-Px, NQO1 and Nrf2 in the liver by 96.96%, 94.26%, 116.71%, 101.51% and 63.01%, respectively (P < 0.01), but decreased the mRNA level of Keap1 by 33.54% (P < 0.01). Conclusion: Mulberry leaf alkaloids have a protective effect on D-Gal-induced oxidative protein damage in mice via adjusting Keap1/Nrf2 signal pathway.

Key words: mulberry leaf alkaloids; D-galactose; protein; oxidative damage; nuclear erythroid related factor 2/Kelch-like ECH-associated protein-1 signaling pathway

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