Determination of Dithianon Residue and Dissipation Dynamics in Apple and Soil by HPLC Coupled with Modified QuEChERS

Abstract

Abstract: A method was developed for the determination of dithianon residues in apple and soil by using high performance liquid chromatography (HPLC). The residues in the samples were extracted by acetonitrile, cleaned up by the developed QuEChERS method, and separated on an Agilent Eclipse XDB-C18 column, then analyzed with HPLC-VWD at detection wavelength of 250 nm. The dithianon residue was quantified by the external standard method using the matched standard solution to compensate for matrix effect. Under the optimal conditions, the calibration curves showed good linearity in the range of 0.02-5 mg/kg for dithianon. The average recoveries of dithianon was in the range of 74% - 92%, with relative standard deviations (RSDs) of 1.02% - 6.20%. The limits of detection (LOD, RSN > 3) of dithianon was 0.005 ng, the limits of quantitation (LOQ) was 0.02 mg/L. The half-life of dithianon were 3.8 - 12.6 d and 1.0 - 8.3 d in apple and soil, respectively. At harvest, apple samples were found to contain dithianon well below the maximum residue limit (MRL) of the China (5 mg/kg) following the recommended dosage and the interval of 14, 21 and 28 days after last application. The harvest interval was recommended to be 14 d.

Key words: dithianon, QuEChERS, high performance liquid chromatography (HPLC), apple, soil, residue

CLC Number:

Xu QIN. Determination of Dithianon Residue and Dissipation Dynamics in Apple and Soil by HPLC Coupled with Modified QuEChERS[J]. FOOD SCIENCE, 0, (): 0-0.

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