Abstract: Casein glycomacropeptide (CGMP) is a kind of peptide that has many physiological functions and can be widely used in functional foods and medicine. Anion exchange resin has been used to separate casein glycomacropeptide from whey powder. The optimal separation conditions of anion exchange resin were investigated to explore the effects of adsorption pH, adsorption time and buffer concentration on separation efficiency of CGMP. The results showed that the appropriate exchange resin was resin 201×4. The optimal adsorption conditions were adsorption buffer pH of 5.1, adsorption time of 1 h, and adsorption buffer concentration of 0.02 mol/L. The optimal elution conditions were elution buffer concentration of 0.5 mol/L, elution time of 1 h and elution buffer pH of 4.0. After removing the impurity from whey powder at pH 5.1 by centrifugation, 1.45 g CGMP with sialic acid concentration of 10.4% was achieved from 100 g whey powder. The whole separation process conditions were simple and the separation efficiency was satisfory so that anion exchange resin is suitable for the industrial production of CGMP.

Key words: casein glycomacropeptide (CGMP), anion exchange resin, whey powder, sialic acid