FOOD SCIENCE ›› 2017, Vol. 38 ›› Issue (15): 201-207.doi: 10.7506/spkx1002-6630-201715033

• Nutrition & Hygiene • Previous Articles     Next Articles

Milk-Derived Casein Glycomacropeptide (CGMP) Regulates Inflammation in Human Colon Carcinoma Cells HT-29

WANG Yong, GONG Jianmiao, JIA Yan, YAN Yali, PANG Guangchang, ZHAO Pei, CHEN Qingsen   

  1. (Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China)
  • Online:2017-08-15 Published:2017-09-06

Abstract: To further elucidate the anti-inflammatory activity of casein glycomacropeptide (CGMP) and its efficacy in enhancing recovery from intestinal inflammation, the effect of CGMP on the NF-κB p65 subunit in colon carcinoma cells HT-29 treated with lipopolysaccharide (LPS) and its role in regulating the NF-κB signaling pathway were determined. Furthermore, plasmids were constructed to express CGMP and NF-κB?essential?modulator (NEMO) and then used to co-transfect HT-29 cells. Co-immunoprecipitation was used to identify the interaction between the expressed proteins. Finally, we detected the pro-apoptotic effect of CGMP on HT-29 cells by measuring the change in mitochondrial transmembrane potential. The experimental results indicated that after 30 h transfection of HT-29 cells, the co-expression and interaction of CGMP and NEMO were confirmed. CGMP affected the NF-κB signaling pathway through its interaction with NEMO with a concomitant increase in apoptosis of HT-29 cells in a time-dependent manner. This effect of CGMP was maximum at 0.1 μg/mL. In conclusion, the interaction of CGMP and NEMO can indirectly affect IKK (IκB kinase) and therefore the NF-κB signaling pathway through IκB. The study revealed another way CGMP could regulate the NF-κB signaling pathway, and fully indicated that there are multiple ways CGMP could execute anti-inflammatory effect.

Key words: casein glycomacropeptide (CGMP), colon carcinoma cells HT-29, NF-κB signaling pathway, NF-κB essential modulator (NEMO), apoptosis

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