FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (5): 93-101.doi: 10.7506/spkx1002-6630-20181227-325

• Nutrition & Hygiene • Previous Articles     Next Articles

RNA-seq Transcriptomic Analysis of Phycocyanin in Regulating the Inhibition of Non-small Cell Lung Cancer Cells

HAO Shuai, LI Shuang, WANG Jing, WU Tingting, ZHANG Jiawen, WANG Chengtao   

  1. (Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, School of Food and Health, Beijing Technology and Business University, Beijing 100048, China)
  • Online:2020-03-15 Published:2020-03-23

Abstract: Phycocyanin is a natural food colorant used in many food industries in China. It is also a natural functional food protein with good antitumor capacity. In the present study, the high-throughput RNA-seq analysis showed that phycocyanin affected the expression levels of 2 532 genes, including 1 491 up-regulated ones and 1 041 down-regulated ones. GO analysis showed that these differentially expressed genes mainly participated in cell proliferation, apoptosis and cytokine responses. Cluster analysis showed that proliferation-related genes such as Cdk2, Cdc25, PCNA and TLR4 were down-regulated, whilst p27 and p21 were up-regulated after phycocyanin treatment. Meanwhile, apoptosis-related genes such as Bcl-2 and NKD1 were significantly down-regulated, whereas CCT6 and GBP2 were significantly up-regulated. KEGG pathway analysis showed that PI3K-Akt signaling was one of the important pathways through which phycocyanin participated in regulating H460 cells. By revealing the potential mechanism of action of phycocyanin in non-small cell lung cancer H460 cells, the present study may provide a theoretical foundation for the development of targeted therapeutics against non-small cell lung cancer and of functional food factors against tumor cells.

Key words: phycocyanin, non-small cell lung cancer, H460 cells, transcriptomic, cell proliferation, cell apoptosis

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