Abstract: Objective: To study the effect of grape seed procyanidin extract (GSPE) combined with cisplatin (cisdichlorodiamineplatinum (II), cDDP) on cell apoptosis and apoptosis-related protein expression in mouse testicular sertoli TM4 cells. Methods: Normal TM4 cells were cultured in vitro and divided into control, cDDP (0.014 mg/mL), GSPE (0.005 mg/mL) + cDDP (0.014 mg/mL) and GSPE (0.005 mg/mL) group. Flow cytometry was used to determine the effect of GSPE on cDDP-induced cell apoptosis rate, Hoechst staining was used to determine the morphological change of TM4 cells, and Western blot was used to determine the protein expression levels of apoptosis-related genes. Results: cDDP significantly increased the apoptosis of TM4 cells, significantly decreased the expression of the anti-apoptosis proteins Bcl-2 and pro-caspase 3, significantly increased the expression of the pro-apoptosis protein Bax, and increased the expression of activated caspase-3. GSPE could significantly reduce cDDP-induced apoptosis, reduce the expression of Bax and caspase-3, and increase the expression of Bcl-2 and pro-caspase3. Conclusion: Apoptosis plays an important role in TM4 cytotoxicity induced by cDDP. Bcl-2, Bax, caspase-3 and other proteins are involved in the cDDP-induced apoptosis of TM4 cells, which can be inhibited by GSPE through inhibiting the protein expression of Bax and caspase-3 and enhancing the expression of Bcl-2 and pro-caspase 3, thereby reducing cDDP-induced damage to TM4 cells.

Key words: grape seed proanthocyanidin extract, cisplatin, mouse testicular sertoli TM4 cells, cell apoptosis