FOOD SCIENCE ›› 2021, Vol. 42 ›› Issue (17): 19-26.doi: 10.7506/spkx1002-6630-20200916-218

• Basic Research • Previous Articles     Next Articles

Structural Characterization and Antioxidant and Immunoregulatory Activity of Glycoproteins from Black Soybeans

HU Yihua, WANG Xingyan, ZHANG Wuxia, YUE Aiqin, DU Weijun, ZHAO Jinzhong, LI Peng   

  1. (1. Basic Department, Shanxi Agricultural University, Taigu 030801, China; 2. College of Agriculture, Shanxi Agricultural University, Taigu 030801, China)
  • Published:2021-09-29

Abstract: In this paper, crude glycoprotein from black soybeans (designated HDJ) prepared by alkaline extraction and then alcohol precipitation was fractionated by column chromatography on DEAE-52 cellulose and Sephadex G-100 into two fractions, designated HDJS2 and HDJS5-2. Their chemical compositions were determined and their structures were characterized by spectroscopic methods. Their antioxidant activities were analyzed by reducing power, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging rate and total antioxidant capacity assays. Immunoregulatory activities were determined by the methyl thiazolyl tetrazolium (MTT) colorimetric method. The results showed that HDJS2 (3.20 × 104 Da) and HDJS5-2 (3.89 × 104 Da) were mainly composed of neutral carbohydrate and protein. The glycopeptide bond types were O-type. HDJS2 was mainly composed of mannose, glucose, galactose, arabinose and fucose together with 96.1% amino acids. HDJS5-2 was mainly composed of mannose, glucose, galactose and arabinose as well as 71.3% amino acids. HDJS2 and HDJS5-2 had antioxidant activity in a dose-dependent. In addition, both glycoproteins could promote splenocyte proliferation, suggesting that they had immunoregulatory effects.

Key words: black soybeans; glycoprotein; antioxidant activity; splenic cell proliferation

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