FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (5): 102-107.doi: 10.7506/spkx1002-6630-20190102-016

• Nutrition & Hygiene • Previous Articles     Next Articles

Alkylresorcinols from Wheat Bran Induced Autophagy and Apoptosis of HepG2 Cells

GUO Yazhou, YANG Xiaoming, LI Yueying   

  1. (1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; 2. School of Medicine, Jiangsu University, Zhenjiang 212013, China)
  • Online:2020-03-15 Published:2020-03-23

Abstract: In this study, two alkylresorcinol homologues C19:0 and C21:0 were isolated by preparative chromatography from wheat bran, and their autophagy and apoptosis-inducing effect on HepG2 cells was studied. The results showed that the structures of alkylresorcinol C19:0 and C21:0 were validated by liquid chromatography-mass spectrometry (LC-MS). Cytotoxicity tests showed that the alkylresorcinols (ARs) at concentrations ranging from 10 to 320 μg/mL could significantly inhibit the growth of HepG2 cells in a dose-dependent manner (P < 0.05, P < 0.01, P < 0.001). Compared with the control group, cell apoptosis rates in the experimental group significantly increased (P < 0.01); the protein expression of cleaved caspase3, Bax/Bcl-2, beclin-1 and LC3II increased but the protein expression of p62 decreased (P < 0.01) after 24 h treatment at 160 μg/mL. Therefore, it was suggested that the alkylresorcinols could induce autophagy in HepG2 cells, and induce apoptosis by activating the caspase-3 pathway, thus inhibiting the growth of HepG2 cells.

Key words: alkylresorcinols, wheat bran, HepG2 cells, apoptosis, autophagy

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