FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (5): 108-114.doi: 10.7506/spkx1002-6630-20190301-010

• Nutrition & Hygiene • Previous Articles     Next Articles

Protective Effect and Mechanism of Fibrinolytic Enzyme from Lyophyllum ulmarium on Alcohol-Induced Injury in Vascular Endothelial Cells

LI Fangfang, CONG He, SHEN Minghua   

  1. (Medical College, Yanbian University, Yanji 133002, China)
  • Online:2020-03-15 Published:2020-03-23

Abstract: Objective: To investigate the protective effect and mechanism of fibrinolytic enzyme from Lyophyllum ulmarium (LUFE) on alcohol-induced injury in human umbilical vein endothelial cells (HUVECs). Methods: Cell viability was evaluated by the methyl thiazolyl tetrazolium assay and the activity of lactate dehydrogenase (LDH) in cell-free culture supernatants was determined. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and the content of malondialdehyde (MDA) were measured by colorimetry. The apoptosis changes of HUVECs were detected with acridine orange/ethidium bromide staining. The level of reactive oxygen species (ROS) and mitochondrial transmembrane potential (Δφm) were evaluated by dihydrorhodamine123 and rhodamine 123 staining, respectively. The protein expression of Bax, Bcl-2, cytochrome c, cleaved cysteinyl aspartate specific proteinase (caspase)-9 and cleaved caspase-3 was determined using Western blot assay. Results: Compared with the model group, LUFE could increase the survival rate, decrease the contents of LDH and MDA, and increase the activity of SOD and GSH-Px in HUVECs. LUFE could also reduce intracellular ROS production, increase mitochondrial transmembrane potential and inhibit apoptosis induced by alcohol. LUFE could down-regulate the expression of cytochrome c, cleaved caspase-9 and cleaved caspase-3, and up-regulate the expression of Bcl-2. Conclusion: LUFE has a protective effect on alcohol-induced injury of HUVECs, which may be related to its antioxidant capacity and its ability to regulate the mitochondrial signal pathway.

Key words: Lyophyllum ulmarium, fibrinolytic enzyme, human umbilical vein endothelial cells, alcohol, protective effect

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