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• Nutrition and Hygiene •     Next Articles

PPurification of millet antioxidant peptide and the resisting effect of the peptide on oxidative stress induced by H2O2 in INS-1 cells

LIU 2, 2, 2, 2, 2,CAO Xiang-Yu 2   

  • Received:2017-08-02 Revised:2018-06-19 Online:2018-10-15 Published:2018-10-24
  • Contact: CAO Xiang-Yu E-mail:xycaolnu@163.com

Abstract: Millet antioxidant peptides were purified with SephadexG-25 and DEAE-32 column chromatography. The cellulose acetate electrophoresis was used to determinate the purity of millet antioxidant peptide. Antioxidant effect were investigated by detecting the scavenging activity of superoxide anion radical, hydroxyl radical and DPPH radical. In order to investigate the protective function of Millet antioxidant peptide, an oxidative stress model of INS-1 cell induced by H2O2 was used. MTT method was applied to detect the cell viability, Flow cytometry was used to detect cell apoptosis, real-time PCR was used to analysis the gene expression of antioxidant enzymes. The results showed that the scavenging rate of O2-? ,?OH and DPPH radical by millet antioxidant peptide purified with column chromatography were 62.71±3.86%、73.56±4.51% and 82.62±5.25%. Millet antioxidant peptide purified by column chromatography can promote the cell viability, reduce the level of reactive oxygen species, and significantly resist the occurrence of apoptosis, the probably mechanism of millet antioxidant protecting INS-1 cells damaged by H2O2 is up-regulating the enzymes expression including SOD1、CAT、GST、NQO1. The millet antioxidant peptide shows significant effect on resisting oxidative stress in INS-1 cells induced by H2O2.

Key words: Millet antioxidant peptide, Oxidative stress, Diabetic cell, Apoptosis

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