Application of Illumina MiSeq High-Throughput Sequencing Technique to Analyze Bacterial Distribution in Raw Milk

doi:10.7506/spkx1002-6630-201816027

Abstract

Abstract: This study was designed to determine the distribution of bacterial populations in raw milk by Illumina MiSeq high-throughput sequencing method. The bacterial diversity and the distribution of pathogenic bacteria in raw milk samples collected from 14 dairy farms were determined by means of DNA sequencing with operational taxonomic unit (OTU) clustering analysis, Alpha diversity analysis and species classification analysis. The results showed that bacterial diversity was found in the milk samples from different farms with the genera Enterococcus, Bacillus, Acinetobacter and Lactococcus being the most diverse. At the same time, Staphylococcus aureus and Shigella were detected in only a few milk samples.

Key words: Illumina MiSeq high-throughput sequencing, raw milk, bacterial distribution

CLC Number:

TS201.1

YU Guoping, CHEN Yuan, YAO Yuxiu, FAN Meijing, LIU Peng, DONG Liangwei. Application of Illumina MiSeq High-Throughput Sequencing Technique to Analyze Bacterial Distribution in Raw Milk[J]. FOOD SCIENCE, 2018, 39(16): 186-191.

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Application of Illumina MiSeq High-Throughput Sequencing Technique to Analyze Bacterial Distribution in Raw Milk

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