Abstract: Atrazine molecularly imprinted polymer microspheres (MIPMs) were prepared by precipitation polymerization with atrazine as template molecule, methacrylic acid (MAA) as functional monomer, acetonitrile as porogen. The preparation processes of the MIPMs were optimized, when atrazine and MAA mole ratio was 1:4, acetonitrile dosage was 50 mL, polymerization temperature was 60°C, the adsorption effect of the MIPMs was best. The adsorption properties of the MIPMs to target substances were investigated through adsorption experiments. Combined with Scatchard analysis, there were two types of adsorption sites for atrazine in the MIPMs, and the maximum apparent binding capacity was 282.69 μmol/g. Molecularly imprinted solid phase extraction (MISPE) column was prepared with the MIPMs as SPE materials for sample pretreatment, and a method was established by atrazine-MISPE-high performance liquid chromatography for determination four triazine pesticide residues (including simazine, ametryn, atrazine and prometryn) in foods. The results showed that the MISPE column had specific selectivity to the four triazine pesticides, and the linear correlation coefficients of the four pesticides were 0.9991～0.9997, the detection limits were 0.5 ng/mL～5 ng/mL, the average recoveries were between 86.2% and 95.7%, and the relative standard deviations were 1.98% ~ 4.51% (n=5). This method can easily, accurately and selectively detect triazine pesticide residues in foods.

Key words: atrazine, triazine pesticide residues, molecularly imprinted polymer microspheres, adsorption properties, precipitation polymerization, solid phase extraction, high performance liquid chromatography, food detection