Simultaneous Determination of Vitamins A, D and E in Foods for Special Medical Purposes by Online Solid Phase Extraction Combined with Two-Dimensional Liquid Chromatography

doi:10.7506/spkx1002-6630-20220923-241

FOOD SCIENCE ›› 2023, Vol. 44 ›› Issue (20): 315-320.doi: 10.7506/spkx1002-6630-20220923-241

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Simultaneous Determination of Vitamins A, D and E in Foods for Special Medical Purposes by Online Solid Phase Extraction Combined with Two-Dimensional Liquid Chromatography

ZHAI Hongwen, MA Hongyu, MA Junmei, CAO Meirong, WANG Jing, WANG Juan, LI Qiang

(Hebei Food Safety Key Laboratory, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Engineering Research Center for Special Food Safety and Health, Hebei Food Inspection and Research Institute, Shijiazhuang 050091, China)

Online:2023-10-25 Published:2023-11-07

Abstract

Abstract: A rapid method for the determination of VA, VD (VD2, VD3) and VE (α-, β-, γ-, and δ-vitamin E) in foods for special medical purposes was established using online solid phase extraction (SPE) combined with two-dimensional liquid chromatography (2D-LC). After saponification, enrichment and purification of VA, VD and VE from samples were accomplished on an online SPE cartridge. VA and four VEs were quantitatively analyzed by the first-dimensional LC, and VD was transferred by heart-cutting to the second-dimensional chromatographic system for separation and detection. SPE columns with different fillings, sample loading solvents, and eluent composition were evaluated, and 2D-LC conditions were systematically optimized. Good linearity was achieved for all vitamins tested with correlation coefficients (R2) greater than 0.997. The mean recoveries of the developed method were 84.4%–109.0% with relative standard deviation (RSD) of 0.34%–6.55% (n = 6). This method was highly automated with low detection errors and high detection efficiency, and could be used for the analysis of vitamins in foods for special medical purposes.

Key words: two-dimensional liquid chromatography; online solid phase extraction; food for special medical purposes; VA; VD; VE

CLC Number:

TS201.2

ZHAI Hongwen, MA Hongyu, MA Junmei, CAO Meirong, WANG Jing, WANG Juan, LI Qiang. Simultaneous Determination of Vitamins A, D and E in Foods for Special Medical Purposes by Online Solid Phase Extraction Combined with Two-Dimensional Liquid Chromatography[J]. FOOD SCIENCE, 2023, 44(20): 315-320.

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Simultaneous Determination of Vitamins A, D and E in Foods for Special Medical Purposes by Online Solid Phase Extraction Combined with Two-Dimensional Liquid Chromatography

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