Determination of Phenethylamine in Fish Sauce by Amperometric I-t Curve Method

Abstract

Abstract: Phenethylamine was determined by integrated pulsed amperometric detection method and amperometric i-t curve method. The detection conditions of the two methods were optimized, and the detection results were compared. The results showed that amperometric i-t curve method had better selectivity and higher sensitivity to phenethylamine based on gold nanoparticles (AuNPs) modified gold electrode (AuE) at the initial potential of 0.1 V. The difference of response current had good linear relationship with the concentration of phenethylamine in a range of 4.13 × 10-8 - 4.72 × 10-6 mol/L. The detection limit was 1.4 × 10-8 mol/L. The recovery of phenethylamine in fish sauce was in the range of 90.0%-110.8%. This detection method was rapid, inexpensive, high-selective, and suitable for determination of phenethylamine in aquatic products and their products.

Key words: amperometric i-t curve, integrated pulsed amperometric detection, phenethylamine, gold nanoparticles, fish sauce

CLC Number:

TS207.3

References

[1]范春艳, 孔维府, 潘秋红, 等.葡萄酒中种生物胺的-检测方法的研究[J].中国酿造, 2010, 29(11):168-171
[2]孔维府, 范春燕, 张翛翰, 等.论葡萄酒中生物胺生成的影响因素及其检测方法[J].中国酿造, 2010, 29(6):13-16
[3]NAL A.A review: Current analytical methods for the determination of biogenic amines in foods[J].Food Chemistry, 2007, 103(4):1475-1486
[4]PIASTA A M, JASTRZEBSKA A, KRZEMINSKI M P, et al.New procedure of selected biogenic amines determination in wine samples by HPLC[J].Analytica Chimica Acta, 2014, 834(1):58-66
[5]GONG X, QI N, WANG X, et al.Ultra-performance convergence chromatography (UPC2) method for the analysis of biogenic amines in fermented foods[J].Food Chemistry, 2014, 162(11):172-175
[6]NAL A, TEKKELI S E, ?NAL C.A review of the liquid chromatographic methods for the determination of biogenic amines in foods[J].Food Chemistry, 2013, 138(1):509-515
[7]陆永梅, 董明盛, 吕欣, 等.高效液相色谱法测定黄酒中生物胺的含量[J].食品科学, 2006, 27(1):196-199
[8]曹利瑞, 朱松, 俞剑燊, 等.黄酒中种生物胺的高效液相色谱分析法[J].食品科学, 2016, 37(4):103-107
[9]ROMANO A, KLEBANOWSKI H, LA GUERCHE S, et al.Determination of biogenic amines in wine by thin-layer chromatographydensitometry[J].Food Chemistry, 2012, 135(3):1392-1396
[10]HUANG J, GAN N, Lv F, et al.Environmentally friendly solid-phase microextraction coupled with gas chromatography and mass spectrometry for the determination of biogenic amines in fish samples[J].Journal of Separation Science, 2016, 39(22):4384-4390
[11]JASTRZEBSKA A, PIASTA A, SZLYK E.Application of ion chromatography for the determination of biogenic amines in food samples[J].Journal of Analytical Chemistry, 2015, 70(9):1131-1138
[12] AN D, CHEN Z Q, ZHENG J C, et al.Determination of biogenic amines in oysters by capillary electrophoresis coupled with electrochemiluminescence[J] [J].Food Chemistry, 2015, 168:1-6
[13]CORTACERO-RAMIREZ S, ARRAEZ-ROMAN D, SEGURA-CARRETERO A, et al.Determination of biogenic amines in beers and brewing-process samples by capillary electrophoresis coupled to laser-induced fluorescence detection[J].Food Chemistry, 2007, 100(1):383-389
[14]LI W, PAN Y, LIU Y, et al.Simultaneous Determination of Eight Typical Biogenic Amines by CZE with Capacitively Coupled Contactless Conductivity Detection[J].Chromatographia, 2014, 77(3-4):287-292
[15]钱勇强, 沈俊, 炳安冬, 等.毛细管电泳-电化学发光联用检测鱼露中苯乙胺[J].中国食品学报, 2013, 13(2):165-170
[16] APETREI I M, APETREI, C.The biocomposite screen-printed biosensor based on immobilization of tyrosinase onto the carboxyl functionalised carbon nanotube for assaying tyramine in fish products[J[J].Journal of Food Engineering, 2015, 149:1-8
[17]APETREI I M, APETREI, C.Amperometric Biosensor Based on Diamine OxidasePlatinum NanoparticlesGrapheneChitosan Modified Screen-Printed Carbon Electrode for Histamine Detection[J].Sensors, 2016, 16(4):422-436
[18]肖艳华, 史妮, 汪俊丽, 等.基于普鲁士蓝双胺氧化酶修饰的生物胺传感器[J].湖南师范大学自然科学学报, 2014, 37(1):37-41
[19]田鑫, 刘红, 李传勇, 等.反相高效液相色谱法同时检测海产品中种生物胺[J].食品安全质量检测学报, 2017, 8(4):1171-1178
[20]赵晓娟, 李艺勤, 白卫东, 等.基于铜离子促水解反应高灵敏电化学检测鸡肉中头孢氨苄残留[J].分析化学, 2016, 44(12):1927-1933
[21]ZHAO X, MAI Z, KANG X, et al.Direct electrochemistry and electrocatalysis of horseradish peroxidase based on clay-chitosan-gold nanoparticle nanocomposite[J].Biosensors and Bioelectronics, 2008, 23(7):1032-1038
[22]HUANG H Z, YUAN Q, YANG X R.Morphology study of gold-chitosan nanocomposites[J].Journal of Colloid and Interface Science, 2005, 282(1):26-31
[23] 中华人民共和国卫生部, 中国国家标准化管理委员会.食品中生物胺含量的测定: GB/T 5009.208-2008[S]. 北京: 中国标准出版社, 2008: 3.
[24]金根娣, 彭生微, 胡效亚.积分脉冲安培法测定苯乙胺[J].应用化学, 2008, 25(12):1404-1408
[25]JHA N, RAMAPRABHU S.Development of Au nanoparticles dispersed carbon nanotube-based biosensor for the detection of paraoxon[J].Nanoscale, 2010, 2(5):806-810
[26]MIN J Z, YAMASHITA K, TOYOOKA T, et al.Simultaneous and group determination methods for designated substances by HPLC with multi-channel electrochemical detection and their application to real samples[J].Biomedical Chromatography, 2010, 24(12):1287-1299
[27]WANG L H, HUANG S J.Design of a Flow-Through Voltammetric Sensor Based on an Antimony-Modified Silver Electrode for Determining Lithol Rubine B in Cosmetics[J].Journal of Automated Methods and Management in Chemistry, 2011, 2011(4):1-7