Abstract Phenethylamine was determined by integrated pulsed amperometric detection method and amperometric i-t curve method. The detection conditions of the two methods were optimized, and the detection results were compared. The results showed that amperometric i-t curve method had better selectivity and higher sensitivity to phenethylamine based on gold nanoparticles (AuNPs) modified gold electrode (AuE) at the initial potential of 0.1 V. The difference of response current had good linear relationship with the concentration of phenethylamine in a range of 4.13 × 10-8 - 4.72 × 10-6 mol/L. The detection limit was 1.4 × 10-8 mol/L. The recovery of phenethylamine in fish sauce was in the range of 90.0%-110.8%. This detection method was rapid, inexpensive, high-selective, and suitable for determination of phenethylamine in aquatic products and their products.