草石蚕中水苏糖提取的脱色工艺研究

doi:10.7506/spkx1002-6300-200920008

食品科学 ›› 2009, Vol. 30 ›› Issue (20 ): 60-63.doi: 10.7506/spkx1002-6300-200920008

草石蚕中水苏糖提取的脱色工艺研究

陈德经¹，韩豪¹，郭婷²

1.陕西理工学院陕西省资源生物重点实验室 2.陕西理工学院生物学院

收稿日期:2009-05-13 出版日期:2009-10-15 发布日期:2010-12-29
通讯作者: 陈德经1， E-mail:cdjslg@126.com
基金资助:
陕西省教育厅资助项目(08JK23)

Decolorization of Stachyose Leached from Chinese Artichoke (Stachys sieboldii Miq.) Tubers

CHEN De-jing1，HAN Hao1，GUO Ting2

1. Bio-resources Key Laboratory of Shaanxi Province, Shaanxi University of Technology, Hanzhong 723000, China ；
2. School of Biological Sciences and Engineering, Shaanxi University of Technology, Hanzhong 723001, China

Received:2009-05-13 Online:2009-10-15 Published:2010-12-29
Contact: CHEN De-jing1 E-mail:cdjslg@126.com

摘要/Abstract

摘要：

为了解决提取草石蚕中水苏糖脱色难问题，对鲜原料分别进行直接打浆法、开水浸烫法、微波法处理，提取后用石灰澄清、磷酸中和、臭氧脱色、柱层析脱色。结果表明：原料处理采用直接微波3min后加水方法较好、经臭氧处理25min后，脱色率可达99.4%。水苏糖提取率5.9%、提取回收率为90.2%，用HPLC检测提取物中水苏糖含量为77.5%。

关键词: 草石蚕, 水苏糖, 脱色, 微波法

Abstract:

Stachyose was leached from Chinese artichoke tubers by a sequence of microwave and beating treatments with the addition of water. In order to achieve high decolorization ratio, the resulting supernatant after centrifugation was subjected to clarification with lime and phosphoric acid (for adjusting pH) and ozone treatment, followed by anion exchange adsorption. After condensation and drying, a final product with 77.5% purity was obtained, which was detected by HPLC. The decolorization ratio was as high as 99.4% when 25 min ozone treatment was conducted for decolorizing supernatant of 3 min microwave-treated Chinese artichoke tubers slurry, and the leaching yield and recovery of stachyose were 5.9% and 90.2%, respectively.

Key words: Chinese artichoke, stachyose, decolorization, microwave

中图分类号:

O636.1

陈德经1，韩豪1，郭婷2. 草石蚕中水苏糖提取的脱色工艺研究[J]. 食品科学, 2009, 30(20 ): 60-63.

CHEN De-jing1，HAN Hao1，GUO Ting2. Decolorization of Stachyose Leached from Chinese Artichoke (Stachys sieboldii Miq.) Tubers[J]. FOOD SCIENCE, 2009, 30(20 ): 60-63.

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草石蚕中水苏糖提取的脱色工艺研究

Decolorization of Stachyose Leached from Chinese Artichoke (Stachys sieboldii Miq.) Tubers

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