关键词: 高效液相色谱, 电喷雾质谱, 氟喹诺酮, 牛肉

Abstract:

A method for analyzing three fluoroquinolone drug residues (ciprofloxacin, enrofloxacin and sarafloxacin) in beef was developed by high performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESI-MS). Samples were extracted with acidified methanol and quantified with the addition of internal standards. An excellent linear was revealed in the range from 1 to 20 pg/μl for three fluoroquinolones with a correlation coefficient of over 0.992. The average spike recovery rates at the levels from 16.7 to 50.0 μg/kg were in the range of 75.2%－96.3% with a relative standard deviation of 2.5%－8.3%. In addition, the detection limit was 8－19 μg/kg, which was much lower than the maximum residue limit (MRL) in beef issued by the Ministry of Agriculture of China, the Department of JECFA and the European Union. This method is simple, rapid and can be used as the reference for the identification and quantification of fluoroquinolone drug residues in edible animal-derived products.

Key words: high performance liquid chromatography, electrospray ionization mass spectrometry, fluoroquinolone, beef