长白山红蚂蚁在体外肿瘤细胞增殖抑制作用研究

doi:10.7506/spkx1002-6300-200921078

食品科学 ›› 2009, Vol. 30 ›› Issue (21 ): 335-337.doi: 10.7506/spkx1002-6300-200921078

长白山红蚂蚁在体外肿瘤细胞增殖抑制作用研究

朴春红¹，杨平²，铃木幸一³，胡耀辉^1,*

1.吉林农业大学食品工程学院 2.日本预防医学研究所 3.岩手大学农学部

收稿日期:2009-06-19 出版日期:2009-11-01 发布日期:2010-12-29
通讯作者: 胡耀辉 E-mail:huyaohui@vip.163.com
基金资助:
吉林农业大学与岩手大学共同合作研究项目

Antitumor Activity of Ethyl Acetate Extract from Formica aquilonia in vitro

PIAO Chun-hong1，YANG Ping2，Suzuki Koichi3，HU Yao-hui1,*

1.College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China ；
2. Graduate Institute of Preventive Medicine, Shizuoka 422-8027, Japan；
3.Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan

Received:2009-06-19 Online:2009-11-01 Published:2010-12-29
Contact: HU Yao-hui E-mail:huyaohui@vip.163.com

摘要/Abstract

摘要：

对长白山红蚂蚁(Forimica aquilonia)的抗肿瘤进行活性研究。MTT 比色法测定长白山红蚂蚁提取物对大鼠肝癌细胞dRLh84 增殖抑制作用，荧光显微镜观察提取物作用于dRLh84 细胞后的肿瘤细胞的细胞核变化。结果表明：长白山红蚂蚁乙酸乙酯提取物具有抑制dRLh84 细胞增殖作用，且呈剂量- 效应关系。乙酸乙酯提物以乙酸乙酯和水进行液液萃取后，活性成分移动至乙酸乙酯相，证明活性物质为极性较小的物质。荧光显微镜观察结果表明，长白山红蚂蚁乙酸乙酯提取物能引起dRLh84 细胞凋亡。

关键词: 长白山红蚂蚁, dRLh84 肿瘤细胞, 增殖抑制

Abstract:

In order to evaluate the antitumor activity of red wood ants (Formica aquilonia) from Changbai mountain, ethyl acetate extract from this species of red wood ants and its ethyl acetate-soluble and water-soluble fractions were investigated for effects on proliferation and apoptosis of dRLh-84 cells using MTT assay and fluoroscopic observation, respectively. The ethyl acetate extract was able to inhibit the proliferation of dRLh-84 cells in a dose-dependent manner. Active components in the extract were transferred into ethyl acetate phase in ethyl acetate-water extraction due to low polarity so that the ethyl acetate phase also exhibited a significant inhibitory effect on the proliferation of dRLh-84 cells, which depended on dose with an IC50 of 60 μg/ml. Fluoroscopic observation indicated that the extract had an obvious apoptosis-promoting effect on dRLh-84 cells.

Key words: Formica aquilonia, dRLh84 tumor cell, proliferation inhibition

中图分类号:

R931.74

朴春红1，杨平2，铃木幸一3，胡耀辉1,*. 长白山红蚂蚁在体外肿瘤细胞增殖抑制作用研究[J]. 食品科学, 2009, 30(21 ): 335-337.

PIAO Chun-hong1，YANG Ping2，Suzuki Koichi3，HU Yao-hui1,*. Antitumor Activity of Ethyl Acetate Extract from Formica aquilonia in vitro[J]. FOOD SCIENCE, 2009, 30(21 ): 335-337.

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长白山红蚂蚁在体外肿瘤细胞增殖抑制作用研究

Antitumor Activity of Ethyl Acetate Extract from Formica aquilonia in vitro

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