食品科学 ›› 2009, Vol. 30 ›› Issue (22 ): 221-225.doi: 10.7506/spkx1002-6300-200922050

• 工艺技术 • 上一篇    下一篇

二次回归正交旋转组合设计在鳀鱼蒸煮液酶解工艺中的应用研究

徐 静1,张 井1,李 燕1,李来好2,薛长湖3   

  1. 1.温州市农业科学研究院食品科学研究所 2.中国水产科学研究院南海水产研究所 3.中国海洋大学食品科学与工程学院
  • 收稿日期:2009-06-23 出版日期:2009-11-15 发布日期:2010-12-29
  • 通讯作者: 徐 静1, E-mail:xujwz@yahoo.com.cn
  • 基金资助:

    国家“863”计划项目(2007AA091801);浙江省科技厅重大科技专项农业项目(2007C12065)

Optimization of Enzymatic Hydrolysis of Anchovy Cooking Waste Using Quadratic Orthogonal Rotation Regression Combination Design

XU Jing1,ZHANG Jing1,LI Yan1,LI Lai-hao2,XUE Chang-hu3   

  1. (1. Institute of Food Science, Wenzhou Academy of Agricultural Sciences, Wenzhou 325006, China;
    2. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;
    3. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China)
  • Received:2009-06-23 Online:2009-11-15 Published:2010-12-29
  • Contact: XU Jing1, E-mail:xujwz@yahoo.com.cn

摘要:

以α- 氨基酸态氮含量为指标,在鳀鱼蒸煮液单因素酶解研究的基础上,采用二次回归正交旋转组合设计对其酶解工艺进行优化。建立酶解液中α- 氨基酸态氮含量与蛋白酶用量、酶解温度及酶解初始pH 值3 个试验因素的正交回归模型方程,根据回归模型进行主效应分析,通过频率分析法得到酶解最佳工艺条件:蛋白酶用量0.56%(m/m),酶解温度50℃,酶解初始pH7.12,最佳条件下酶解液中α- 氨基酸态氮的含量为0.49g/100ml。

关键词: 鳀鱼蒸煮液, 二次回归正交旋转组合设计, 酶解

Abstract:

Anchovy cooking waste was hydrolyzed using proteases, and the effects of proteases concentration, hydrolysis temperature and initial pH on the enzymolysis degree were studied (evaluated by α-amino nitrogen concentration). The enzymatic hydrolysis process was optimized by conducting quadratic orthogonal rotation regression combination design test. The optimum conditions were that anchovy cooking waste was hydrolyzed with 0.56% (m/m) proteases at 50 ℃ with the initial pH 7.12. Under such conditions, the α-amino nitrogen concentration in enzymatic hydrolysis liquid was 0.49 g/100 ml.

Key words: anchovy cooking waste, quadratic orthogonal rotation regression combination design, enzymatic hydrolysis

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