食品科学 ›› 2009, Vol. 30 ›› Issue (23 ): 401-405.doi: 10.7506/spkx1002-6300-200923090

• 营养卫生 • 上一篇    下一篇

富硒大麦苗对小鼠免疫功能的调节

肖颜颜1,王晓洁1 ,* ,戴小曼1,刘新生2,曲彦清2,杜 蓉1,李勤钰1,张 龙1   

  1. 1.鲁东大学生命科学学院 2.鲁东大学校医院
  • 收稿日期:2009-08-13 出版日期:2009-12-01 发布日期:2010-12-29
  • 通讯作者: 王晓洁 E-mail:wxj10304@126.com
  • 基金资助:

    烟台市科学技术发展计划项目(2007152)

Immune Regulation Function of Selenium-enriched Barley Seedling in Mice

XIAO Yan-yan1,WANG Xiao-jie1,*,DAI Xiao-man1,LIU Xin-sheng2,QU Yan-qing2,DU Rong1,LI Qin-yu1,ZHANG Long1   

  1. 1. College of Life Science, Ludong University, Yantai 264025, China;
    2. College Hospital, Ludong University, Yantai 264025, China
  • Received:2009-08-13 Online:2009-12-01 Published:2010-12-29
  • Contact: WANG Xiao-jie E-mail:wxj10304@126.com

摘要:

目的:通过测定小鼠细胞免疫与体液免疫,来研究富硒大麦苗对小鼠免疫功能的调节作用。方法:用不同剂量富硒大麦苗(0.1、0.5、1g/ml)对小鼠进行灌胃,同时设正常大麦苗组(1g/ml)、生理盐水组对照组,各组灌胃量为每天0.2ml。测小鼠的白细胞总数、小鼠腹腔巨噬细胞吞噬功能、抗体效价、抗体生成细胞数量以及免疫器官重量等指标,并对其进行统计分析。结果:富硒大麦苗组与生理盐水组比较能显著提高小鼠腹腔巨噬细胞的吞噬能力(P < 0.01),能明显提高抗鸡红细胞抗体的效价(P < 0.05),明显提高脾脏指数(P < 0.05),明显提高小鼠白细胞总数(P < 0.05)。结论:富硒大麦苗有显著提高小鼠细胞免疫与体液免疫的功能,并与其剂量呈正相关。

关键词: 富硒大麦苗, 免疫功能, 小鼠

Abstract:

Objective: To observe immune regulation function of selenium-enriched barley seedling (SEBS) in mice by detecting cell-mediated immunity and humoral immunity. Methods: Experimental mice were orally administered with SEBS at doses of 1, 0.5 g/ml and 0.1 g/ml. Meanwhile, two groups were also orally administered with saline water at the same volume and regular barley seedling at the dose of 1 g/ml as the controls. Total number of white blood cell (WBC), phagocytosis test of macrophage on abdominal cavity, titer of antibody, number of antibody-producing cells and weight of immune organs in mice were determined. Results: Compared to saline water group, SEBS could significantly improve phagocytic function of macrophages (P<0.01), obviously increase the titer of anti-CRBC antibody (P<0.05), spleen index (P<0.05), and total number of WBC (P<0.05). Conclusion: SEBS can promote cell-mediated immunity and humoral immunity in mice in a dosedependent manner.

Key words: selenium-enriched barley seedling, immune function, mice

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