反应时间对DPPH·法、ABTS+·法评价抗氧化性结果的影响

doi:10.7506/spkx1002-6300-201005015

食品科学 ›› 2010, Vol. 31 ›› Issue (5): 63-67.doi: 10.7506/spkx1002-6300-201005015

反应时间对DPPH·法、ABTS+·法评价抗氧化性结果的影响

林恋竹，赵谋明*

华南理工大学轻工与食品学院

收稿日期:2009-07-01 修回日期:2009-11-09 出版日期:2010-03-01 发布日期:2010-12-29
通讯作者: 赵谋明 E-mail:femmzhao@scut.edu.cn
基金资助:
广东省科技计划项目(2007A020902002)

Effect of Reaction Time on DPPH and ABTS+ Radical Scavenging Assays for Antioxidant Capacity Evaluation

LIN Lian-zhu，ZHAO Mou-ming*

College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China

Received:2009-07-01 Revised:2009-11-09 Online:2010-03-01 Published:2010-12-29
Contact: ZHAO Mou-ming E-mail:femmzhao@scut.edu.cn

摘要/Abstract

摘要：

由于DPPH·法、ABTS+·法操作简单，不需要特殊的检测设备(通过反应体系颜色的改变评价试样抗氧化性)，因此，常被选作抗氧化性能评价方法。用这两种方法进行抗氧化性评价时，通常用固定反应时间下算得的EC50 值(自由基清除率为50% 时所需试样浓度)表征抗氧化剂的抗氧化性强弱(对于DPPH·法，固定时间为30min；对于ABTS+·法，固定时间为6min)，结果表明：不同抗氧化剂因其组成、结构的差异，与DPPH·、ABTS+·反应速率不同，反应到达平衡的时间不同，将反应时间固定在某一值时，可能对抗氧化剂的抗氧化性评价带来错误的判断。

关键词: 反应时间, DPPH·法, ABTS+·法, 抗氧化性评价

Abstract:

Due to the operation simplicity of DPPH and ABTS+ radical scavenging arrays for evaluating antioxidant capacity and without a need for specific equipments, both assays have been widely chosen to evaluate antioxidant capacity. EC50 is generally used as a representative parameter for antioxidant capacity of antioxidants determined using DPPH· assay at 30 min of fixed reaction time or using ABTS+· assay at 6 min of fixed reaction time. The results of this investigation indicated that a big difference was made by the compositions and structures of antioxidants to reaction rates with DPPH·and ABTS+·free radicals. The reaction equilibrium time was also different so that wrong estimations might be achieved at the condition of fixed reaction time.

Key words: reaction time, DPPH radical scavenging array, ABTS+ radical scavenging array, antioxidant capacity evaluation

中图分类号:

TS202.3

林恋竹，赵谋明*. 反应时间对DPPH·法、ABTS+·法评价抗氧化性结果的影响[J]. 食品科学, 2010, 31(5): 63-67.

LIN Lian-zhu，ZHAO Mou-ming*. Effect of Reaction Time on DPPH and ABTS+ Radical Scavenging Assays for Antioxidant Capacity Evaluation[J]. FOOD SCIENCE, 2010, 31(5): 63-67.

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反应时间对DPPH·法、ABTS+·法评价抗氧化性结果的影响

Effect of Reaction Time on DPPH and ABTS+ Radical Scavenging Assays for Antioxidant Capacity Evaluation

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