食品科学 ›› 2009, Vol. 30 ›› Issue (6): 213-215.doi: 10.7506/spkx1002-6630-200906048

• 分析检测 • 上一篇    下一篇

青藏地区青稞中VE 的高效液相色谱测定

张春红1,2,许传梅1,2,董 琦1,胡凤祖1,*   

  1. 1. 中国科学院西北高原生物研究所
    2.中国科学院研究生院
  • 收稿日期:2008-04-20 修回日期:2008-08-08 出版日期:2009-03-15 发布日期:2010-12-29
  • 通讯作者: 胡凤祖 E-mail:hufz@nwipb.ac.cn

HPLC Determination of Vitamin E Content in Highland Barley from Tibetan Plateau

ZHANG Chun-hong1,2,XU Chuan-mei1,2,DONG Qi1,HU Feng-zu1,*   

  1. (1. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China;
    2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China)
  • Received:2008-04-20 Revised:2008-08-08 Online:2009-03-15 Published:2010-12-29
  • Contact: HU Feng-zu1,* E-mail:hufz@nwipb.ac.cn

摘要:

采用高效液相色谱法对青藏高原的16 种不同地区和品种的青稞中δ-VE、γ- VE 和α- VE 同时进行了含量的测定,为青稞的质量评价和综合利用提供依据。采用Symmetry C18(4.6mm × 250mm,5μm)色谱柱;甲醇:水=98:2(V/V)为流动相;检测波长295nm;柱温25℃;流速1ml/min。结果表明,青藏地区的青稞中VE 总含量在0.3~0.8mg/100g,其中青海地区青稞中VE 的平均总含量比西藏地区低约10%。

关键词: 高效液相色谱(HPLC), 青稞, 维生素E

Abstract:

In order to provide scientific basis for the quantity control and comprehensive utilization of highland barley, δ-vitamin E, γ- vitamin E , α- vitamin E contents in 16 cultivars of highland barley from Qinghai and highland barley samples from different 10 areas of Tibet were determined by HPLC. The chromatographic column, mobile phase, column temperature, flow rate and detection wavelength were Symmetry C18 column (4.6 mm × 250 mm, 5 μm), methanol-water at the volume ratio of 98: 2, 25 ℃, 1 ml/min and 295 nm, respectively. The results indicated that all the collected highland barley in Tibetan plateau contain vitamin E,and the total content is between 0.3 mg/100g and 0.8 mg/100 g, especially the average content of total vitamin E in highland barley from Tibet is higher about 10% than that from Qinghai.

Key words: HPLC, highland barley, VE

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