食品科学 ›› 2009, Vol. 30 ›› Issue (7): 177-180.doi: 10.7506/spkx1002-6630-200907040

• 生物工程 • 上一篇    下一篇

低能氮离子注入诱变选育乳链菌肽产生菌的研究

陈洪卫,陈林海*,张国只   

  1. 郑州大学 河南省离子束生物工程重点实验室
  • 收稿日期:2008-06-06 出版日期:2009-04-01 发布日期:2010-12-29
  • 通讯作者: 陈林海 E-mail:chenlinhai@zzu.edu.cn
  • 基金资助:

    国家“973”计划项目(2004CB719604)

Study on Mutagenesis of Nisin-producing Strain Induced by Low Energy N+ Implantation

CHEN Hong-wei CHEN Lin-hai* ZHANG Guo-zhi   

  1. (Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, Zhengzhou 450052, China)
  • Received:2008-06-06 Online:2009-04-01 Published:2010-12-29
  • Contact: CHEN Lin-hai* E-mail:chenlinhai@zzu.edu.cn

摘要:

本实验以低能氮离子和紫外线作为诱变手段对乳链菌肽产生菌进行诱变,筛选出一株优良菌株Lactococcus lactis K25,发酵效价由初始的1732.45 IU/ml提高到2291.75 IU/ml,提高了32.3%。遗传稳定性实验结果表明,Lactococcus lactis K25遗传性状稳定良好。

关键词: 乳链菌肽, 离子束注入, 诱变

Abstract:

In this study, the nisin-producing strain Lactococcus lactis J7 was mutagenized using low energy N+ implantation and UV radiation, and its mutant strain Lactococcus lactis K25 was obtained. The nisin potency is increased from 1732.45 IU/ml to 2291.75 IU/ml, by 32.3% through the mutagenesis of Lactococcus lactis J7. Moreover, Lactococcus lactis K25 shows nice genetic stability.

Key words: nisin, ion beam implantation, mutagenesis

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