食品科学 ›› 2009, Vol. 30 ›› Issue (24 ): 34-37.doi: 10.7506/spkx1002-6630-200924004

• 工艺技术 • 上一篇    下一篇

紫花杜鹃干燥嫩叶中黄酮类化合物的提取及膜分离纯化

陈 玲1,李炳辉1,张志平1,别平平1 , 2,蒲华寅1,李晓玺1   

  1. 1.华南理工大学轻工与食品学院,淀粉与植物蛋白深加工教育部工程中心 2.佛山市华昊华丰淀粉有限公司
  • 收稿日期:2009-06-23 出版日期:2009-12-15 发布日期:2010-12-29
  • 通讯作者: 陈玲 E-mail:felchen@scut.edu.cn
  • 基金资助:

    “十一五”国家科技支撑计划项目(2006BAD27B04)

Extraction and Purification of Flavonoids from Rhododendoni mariae Hance

CHEN Ling1,LI Bing-hui1,ZHANG Zhi-ping1,BIE Ping-ping1,2,PU Hua-yin1,LI Xiao-xi1   

  1. 1. College of Light Industry and Food Sciences, South China University of Techonology, Engineering Research Center of Starch and Plant
    Protein Processing, Ministry of Education, Guangzhou 510640, China;2. Foshan Huahao Huafeng Co. Ltd., Foshan 528000, China
  • Received:2009-06-23 Online:2009-12-15 Published:2010-12-29
  • Contact: CHEN Ling1, E-mail:felchen@scut.edu.cn

摘要:

在单因素试验的基础上,利用正交试验法优化紫花杜鹃干燥嫩叶片中黄酮类化合物的工艺条件,得到最佳条件为:紫花杜鹃干燥嫩叶片切片浸泡2h,液料比12:1(ml/g),提取3 次,每次1h,并在此基础上,利用膜分离技术对其进行纯化研究。结果表明,将提取液依次通过截留相对分子质量为105、5 × 104、104 的膜后,其纯度可达到46.07%。

关键词: 紫花杜鹃, 黄酮类化合物, 膜分离技术, 纯化

Abstract:

In this study, the extraction of flavonoids from Rhododendoni mariae Hance was optimized by the single factor experiments and orthogonal experiment design. The optimized parameters were obtained as follows: 2 h of presoaking of medicinal materials slices, the liquid/solid of 12:1 (ml/g), extracted 3 times and 1 h of each extraction time. The membrane separation technology was applied to purify samples based on these results. The results showed that the purity of the fraction each 46.07% through relative molecular mass 105, 5×104 and 104 filters.

Key words: Rhododendoni mariae Hance, flavonoids, membrane separation, purification

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