食品科学 ›› 2010, Vol. 31 ›› Issue (6): 127-130.doi: 10.7506/spkx1002-6630-201006028

• 工艺技术 • 上一篇    下一篇

正交试验法优选鹰嘴豆种子中鹰嘴豆芽素A的提取工艺

田春元   

  1. 孝感学院生命科学技术学院
  • 收稿日期:2009-03-02 出版日期:2010-03-15 发布日期:2010-12-29
  • 通讯作者: 田春元 E-mail:hbtcy@yahoo.com.cn

Orthogonal Array Design for Optimization of Extraction of Biochanin A from Chickpea Seed

TIAN Chun-yuan   

  1. School of Life Science and Technology, Xiaogan University, Xiaogan 432000, China
  • Received:2009-03-02 Online:2010-03-15 Published:2010-12-29
  • Contact: TIAN Chun-yuan E-mail:hbtcy@yahoo.com.cn

摘要:

目的:优选鹰嘴豆种子中鹰嘴豆芽素A 的提取工艺。方法:采用正交试验法,以鹰嘴豆种子中鹰嘴豆芽素A的含量为指标进行试验。 结果:优选出从鹰嘴豆种子中提取鹰嘴豆芽素A的最佳工艺:10 倍于生药量的95%乙醇溶液热回流提取两次,每次1.5h。 结论:该工艺可提高鹰嘴豆种子中鹰嘴豆芽素A 的提取率和纯度,而且方便、安全、无毒、成本低、效率高,可在大规模生产鹰嘴豆芽素A 时推广应用。另外,本研究还建立了一种新的快速、准确检测鹰嘴豆种子中鹰嘴豆芽素A 的RP-HPLC 方法。

关键词: 鹰嘴豆种子, 鹰嘴豆芽素A, 正交试验, RP-HPLC

Abstract:

Orthogonal array design was used to optimize hot ethanol reflux extraction of biochanin A from chickpea (Cicer arietinium L.) seed in this study. Results showed that an optimal compromise between biochanin A yield and purity was achieved by two-time extraction at a material/liquid ratio of 1:10 for 1.5 h each time. This extraction method, thanks to its convenience, safety, low cost and high efficiency, can be popularized for the large-scale production. Moreover, based on optimized hot ethanol reflux extraction, a RP-HPLC method was developed for rapid and accurate determination of biochanin A from chickpea seed.

Key words: chickpea (Cicer arietinium L.) seed, biochanin A, orthogonal array design, RP-HPLC

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