食品科学 ›› 2010, Vol. 31 ›› Issue (15): 117-120.doi: 10.7506/spkx1002-6630-201015026

• 基础研究 • 上一篇    下一篇

双孢蘑菇多酚氧化酶交联作用对酪蛋白乳化性及其稳定性的影响

吴进菊1,2,高金燕1,3,刘 潇1,2,程 伟1,2,陈红兵1,2,*   

  1. (1.南昌大学 食品科学与技术国家重点实验室
    2.南昌大学 中德联合研究院 3.南昌大学生命科学与食品工程学院
  • 收稿日期:2010-04-23 出版日期:2010-08-15 发布日期:2010-12-29
  • 通讯作者: 陈红兵 E-mail:chbgjy@hotmail.com
  • 基金资助:

    国家“863”计划项目(2006AA10Z324);江西省教育厅科学技术研究项目(GJJ09063);
    江西省研究生创新资金项目

Emulsify Capacity and Stability of Cross-linked Casein with Polyphenol Oxidase from Agaricus bisporus

WU Jin-ju1,2,GAO Jin-yan1,3,LIU Xiao1,2,CHENG Wei1,2,CHEN Hong-bing1,2,*   

  1. 1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;2. Sino-German Joint
    Research Institute, Nanchang University, Nanchang 330047, China;3. School of Life Sciences and Food Engineering, Nanchang
    University, Nanchang 330047, China
  • Received:2010-04-23 Online:2010-08-15 Published:2010-12-29
  • Contact: CHEN Hong-bing1,2,* E-mail:chbgjy@hotmail.com

摘要:

利用双孢蘑菇多酚氧化酶(PPO)对酪蛋白进行交联,考察交联后酪蛋白乳化性和乳化稳定性,并采用SDSPAGE电泳检测交联物的形成情况。结果表明:当PPO 酶比活力为400U/mL,25℃反应4h 时,交联后的酪蛋白乳化性和乳化稳定性分别为18.3m2/g 和19.8min,较未交联的酪蛋白乳化性和乳化稳定性分别提高了17.3% 和37.5%。交联后酪蛋白的乳化性和乳化稳定性得到显著提高,并且与形成的聚合物含量有着密切的关系。

关键词: 双孢蘑菇多酚氧化酶, 酪蛋白, 交联, 乳化性, 稳定性

Abstract:

Cross-linking of food proteins can modify their functional properties. Casein was cross-linked by polyphenol oxidase from Agaricus bisporus. The emulsifying capacity and emulsion stability of cross-linked casein were analyzed and SDS-PAGE was used to detect the amount of polymer formation. The emulsifying capacity and emulsion stability of cross-linked casein obtained after polyphenol oxidase treatment at 25 ℃ and an enzyme concentration of 400 U/mL for 4 h were 18.3 m2/g and 19.8 min, 17.3% and 37.5% higher than those of non-cross-linked casein, respectively. Such significant increments in both emulsifying properties might be tightly related to the amount of formed cross-linked casein.

Key words: Agaricus bisporus polyphenol oxidase, casein, cross-linking, emulsifying capacity, emulsion stability

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