N+注入诱变选育混合糖发酵L-乳酸高产菌株

doi:10.7506/spkx1002-6630-201021056

食品科学 ›› 2010, Vol. 31 ›› Issue (21): 248-253.doi: 10.7506/spkx1002-6630-201021056

N+注入诱变选育混合糖发酵L-乳酸高产菌株

庞锐，潘丽军* ，姜绍通，吴学凤

合肥工业大学生物与食品工程学院

收稿日期:2010-08-18 修回日期:2010-10-13 出版日期:2010-11-15 发布日期:2010-12-29
通讯作者: 潘丽军 E-mail:panlijun1955@163.com
基金资助:
国家“863”计划项目(2007AA10Z361)；安徽省自然科学基金项目(090411015)

Breeding of High-yield L-Lactic Acid Strains for Co-fermentation of Glucose and Xylose by N+ Implantation

PANG Rui，PAN Li-jun*，JIANG Shao-tong，WU Xue-feng

School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China

Received:2010-08-18 Revised:2010-10-13 Online:2010-11-15 Published:2010-12-29
Contact: PAN Li-jun E-mail:panlijun1955@163.com

摘要/Abstract

摘要：

采用低能N+ 注入技术对米根霉As3.819 进行诱变选育，以提高该菌株利用混合糖(葡萄糖、木糖)发酵生产L- 乳酸的能力。实验结果表明，菌株的存活率曲线呈典型的“马鞍型”，在注入剂量为50 × 2.5 × 1013ions/cm2时具有较高的正突变率。选育获得突变株N50-7，其L- 乳酸产量为79.42g/L，比出发菌株提高了17.75%，且遗传稳定性较好。对突变株N50-7 的发酵培养基进行了初筛，在混合糖150g/L(葡萄糖100g/L、木糖50g/L)、(NH4)2SO43.0g/L、KH2PO4 0.3g/L、MgSO4·7H2O 0.3g/L、ZnSO4·7H2O 0.4g/L 的条件下发酵72h，L- 乳酸产量最高达到103.81g/L，较初筛前提高了30.71%。

关键词: 离子注入, 米根霉, 诱变育种, 混合糖, L - 乳酸

Abstract:

In order to increase the production of L-lactic acid by co-fermentation of glucose and xylose, Rhizopus oryzae As3.819 was mutated by low energy N+ implantation. The results showed that the survival rate curve was a typical saddle shape with a high positive mutation rate at a dose of 50×2.5×1013 ions/cm2. Under this implantation dose, strain N50-7 was obtained with high yield and stability. L-lactic acid yield by N50-7 was 79.42 g/L, 17.75% higher than the original strain. The optimal composition of fermentation medium for N50-7 were as follows: glucose 100 g/L, xylose 50 g/L, (NH4)2SO4 3 g/L, KH2PO4 0.3 g/L, MgSO4·7H2O 0.3 g/L and ZnSO4·7H2O 0.4 g/L. The final concentration of L-lactic acid was 103.81g/L after 72 h fermentation, which was 30.71% higher than that before breeding.

Key words: ion implantation, Rhizopus oryzae, mutation breeding, mixed sugars, L-lactic acid

中图分类号:

Q815

庞锐，潘丽军*,姜绍通，吴学凤. N+注入诱变选育混合糖发酵L-乳酸高产菌株[J]. 食品科学, 2010, 31(21): 248-253.

PANG Rui，PAN Li-jun*，JIANG Shao-tong，WU Xue-feng. Breeding of High-yield L-Lactic Acid Strains for Co-fermentation of Glucose and Xylose by N+ Implantation[J]. FOOD SCIENCE, 2010, 31(21): 248-253.

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N+注入诱变选育混合糖发酵L-乳酸高产菌株

Breeding of High-yield L-Lactic Acid Strains for Co-fermentation of Glucose and Xylose by N+ Implantation

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