食品科学 ›› 2010, Vol. 31 ›› Issue (21): 373-377.doi: 10.7506/spkx1002-6630-201021085

• 营养卫生 • 上一篇    下一篇

富硒沼泽红假单胞菌G3 菌与牛初乳复配改善小鼠免疫功能的作用研究

杨启银1,2 ,3,王家芳1,贾秉晟1,管海华1,刘 青1   

  1. 1. 南京师范大学生命科学学院 2. 南京市微生物工程技术研究中心 3. 江苏省微生物资源工程化技术研究中心
  • 收稿日期:2010-06-25 出版日期:2010-11-15 发布日期:2010-12-29
  • 通讯作者: 杨启银 E-mail:yangqiyin@163.com
  • 基金资助:

    江苏省科学技术厅高新技术项目(BG2005326)

Combined Effect of Bovine Colostrumson and Selenium-enriched Rhodopseudomonas palustris G3 on Immune Function in Mice

YANG Qi-yin1,2,3,WANG Jia-fang1,JIA Bing-sheng1,GUAN Hai-hua1,LIU Qing1   

  1. 1. College of Life Science, Nanjing Normal University, Nanjing 210046, China;
    2. Nanjing Engineering and Technology Research Center for Microbiology, Nanjing 210046, China;
    3. Jiangsu Engineering and Technology Research Center for Microbial Resources, Nanjing 210046, China
  • Received:2010-06-25 Online:2010-11-15 Published:2010-12-29
  • Contact: YANG Qi-yin E-mail:yangqiyin@163.com

摘要:

目的:探讨富硒沼泽红假单胞菌G3 菌(简称富硒G3 菌)与牛初乳复配对小鼠免疫功能的影响。方法:将富G3 菌与牛初乳复配形成富硒牛初乳,用环磷酰胺构建小鼠免疫低下模型,对免疫低下小鼠灌胃富硒G3 菌、牛初乳和富硒牛初乳,50d 后测定各组小鼠脏器指数、白细胞数、T 淋巴细胞阳性率、血清SOD 酶活性、全血GSH-Px活性,同时计算平均每日进食饲料质量与体质量增加量的比值。结果:富硒G3 菌、牛初乳、富硒牛初乳均能提高免疫低下小鼠对饲料的利用率,能提高免疫低下小鼠SOD、GSH-Px 活性、白细胞数和T 淋巴细胞阳性率,其中富硒牛初乳高剂量组效果最为明显,说明富硒牛初乳高剂量组能显著增强小鼠的免疫功能。

关键词: 硒, 牛初乳, 沼泽红假单胞菌, 环磷酰胺, 免疫功能

Abstract:

In order to investigate the effect of selenium-enriched bovine colostrumson on immune function of immunodepressed mice induced by cyclophosphamide, selenium-enriched Rhodopseudomonas palustris G3 (hereinafter called selenium-enriched G3) was mixed with bovine colostrumson to prepare selenium-enriched bovine colostrumson. The immunodepressed mice were treated with selenium-enriched G3, bovine colostrumson and selenium-enriched bovine colostrumson, respectively. The administration was carried out once a day for 47 successive days after 3 days of adaptive feeding, and organ index, white blood cell number, T-cell population, the contents of SOD and GSH-Px in blood, and feed/gain ratio of mice were determined at the end of the administration. Results indicated that selenium-enriched G3, bovine colostrumson and selenium-enriched bovine colostrumson could increase the utilization efficiency of diet, enhance the activities of SOD and GSH-Px in blood, and improve mouse immune function. Compared with the control group, the selenium-enriched bovine colostrumson exhibited best improved immune at the high dosage.

Key words: selenium, bovine colostrumson, Rhodopseudomonas palustris, cyclophosphamide, immunization

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