食品科学 ›› 2010, Vol. 31 ›› Issue (22): 59-63.doi: 10.7506/spkx1002-6630-201022013

• 工艺技术 • 上一篇    下一篇

响应面法优化微波提取野菊花抑菌物质工艺

蒋 雨1,陈安均1,于 新2,*,褚福红3,赵美美2   

  1. 1.四川农业大学食品学院 2.仲恺农业工程学院轻工食品学院 3.安徽农业大学茶与食品科技学院
  • 收稿日期:2010-02-01 修回日期:2010-06-04 出版日期:2010-11-25 发布日期:2010-12-29
  • 通讯作者: 于 新 E-mail:yuxin1959@yahoo.com.cn

Microwave-assisted Extraction of Antibacterial Substances from Inflorescence of Chrysanthemum lavandulaefolium (Fisch.) Mak.

JIANG Yu1,CHEN An-jun1,YU Xin2,*,CHU Fu-hong3,ZHAO Mei-mei2   

  1. 1. College of Food Science, Sichuan Agricultural University, Ya’an 625014, China ;
    2. College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;
    3. College of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036, China
  • Received:2010-02-01 Revised:2010-06-04 Online:2010-11-25 Published:2010-12-29
  • Contact: YU Xin E-mail:yuxin1959@yahoo.com.cn

摘要:

以野菊花的乙醇提取物对金黄色葡萄球菌的抑菌圈直径为响应值,在单因素试验基础上,根据Box-Behnken试验设计原理,研究响应面法(RSM)优化微波辅助提取野菊花抑菌物质的工艺参数。得到乙醇体积分数为50%,最佳参数为液固比40:1(mL/g)、提取时间15min、微波功率480W。经验证在此条件下,野菊花提取物对金黄色葡萄球菌的抑菌圈直径达到27.02mm,与预测值24.56mm 差异不显著,置信区间大于95%。

关键词: 野菊花, RSM, Box-Behnken 试验设计, 微波提取, 抑菌

Abstract:

Box-Behnken experimental design coupled with response surface analysis was employed to optimize the microwaveassisted extraction of antibacterial substances using 50% aqueous ethanol form the inflorescence of Chrysanthemum lavandulaefolium (Fisch.) Mak. Anti-Staphylococcus aureus activity of the extract was investigated by determining the diameter of inhibition zone. The optimal conditions of microwave extraction were as follows: liquid-to-solid ratio 40:1(mL/g), microwave treatment time 15 min, microwave power 480 W. The experimental diameter of inhibition zone was 27.02 mm, which was in accordance with the predicted value of 24.56 mm.

Key words: Chrysanthemum lavandulaefolium (Fisch.)Mak., RSM, Box-Behnken, microwave extraction, antibacteria

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