食品科学 ›› 2010, Vol. 31 ›› Issue (22): 336-339.doi: 10.7506/spkx1002-6630-201022073

• 分析检测 • 上一篇    下一篇

奶酪及乳清中β-内酰胺酶的检测

刘晓川,喻东威,赵 源,刘志楠,薛志清,李 梅   

  1. 内蒙古蒙牛乳业(集团)股份有限公司
  • 收稿日期:2009-10-12 出版日期:2010-11-25 发布日期:2010-12-29
  • 通讯作者: 刘晓川 E-mail:xiaochuan54613@163.com

Detection and Analysis of β-Lactamase in Cheese and Whey

LIU Xiao-chuan,YU Dong-wei,ZHAO Yuan,LIU Zhi-nan,XUE Zhi-qing,LI Mei   

  1. Inner Mongolia Mengniu Dairy (Group) Co. Ltd., Hohhot 011500, China
  • Received:2009-10-12 Online:2010-11-25 Published:2010-12-29
  • Contact: LIU Xiao-chuan E-mail:xiaochuan54613@163.com

摘要:

为探讨原奶中β- 内酰胺酶在制作奶酪过程中的去向,向无抗无酶的原奶中添加β- 内酰胺酶,使其终水平为50U/mL,按照工艺制作原生奶酪,用E50 法和杯碟法检测奶酪和乳清中的β- 内酰胺酶。结果显示,奶酪中β- 内酰胺酶检测结果为阴性,乳清中β- 内酰胺酶检测结果为阳性,β- 内酰胺酶在奶酪制作中主要残留在乳清中。

关键词: 奶酪, 乳清, β-内酰胺酶, E50法, 杯碟法

Abstract:

To explore the destination of the β-lactamases of raw milk in the process of cheese, antibiotics and β-lactamasefree raw milk was mixed with β-lactamases to a final concentration of 50 U/mL. Then the raw milk was processed to cheese according to the general procedure, and β-lactamase in the cheese and whey was determined by using E50 method and Oxford cup method. The result of β-lactamase is negative in the cheese and positive in the whey. So β-lactamase is mainly left in whey in the processing of cheese.

Key words: cheese, whey, β-lactamase, E50 method, Oxford cup method

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