微波辐照诱变选育高产橙色素红曲霉菌

doi:10.7506/spkx1002-6630-201023050

食品科学 ›› 2010, Vol. 31 ›› Issue (23): 224-227.doi: 10.7506/spkx1002-6630-201023050

微波辐照诱变选育高产橙色素红曲霉菌

徐伟，范志诚，刘艳华

哈尔滨商业大学食品工程学院

收稿日期:2010-09-21 修回日期:2010-11-10 出版日期:2010-12-15 发布日期:2010-12-29
通讯作者: 徐伟 E-mail:xuwei20070808@yahoo.com.cn
基金资助:
黑龙江省科技厅重大科技攻关项目(GA07B401-3)

Mutation Breeding of Monascus purpureus with High Productivity of Orange Pigments by Microwave Irradiation

XU Wei，FAN Zhi-cheng，LIU Yan-hua

College of Food Engineering, Harbin University of Commerce, Harbin 150076, China

Received:2010-09-21 Revised:2010-11-10 Online:2010-12-15 Published:2010-12-29
Contact: XU Wei E-mail:xuwei20070808@yahoo.com.cn

摘要/Abstract

摘要：

采用带水循环冷却装置的微波设备，对紫红曲(Monascus purpureus)进行诱变处理，研究微波辐照功率和辐照时间对红曲霉菌的致死规律和突变规律，获得微波功率和时间的最佳辐照剂量，结果表明：在微波功率500W、辐照时间80s 时，得到突变株W5S8，液态发酵产橙色素色价为16.38U/mL，较原始菌株橙色素色价10.26U/mL 提高了59.62%，连续遗传5 代，产色素性状稳定。

关键词: 红曲霉菌, 微波辐照, 橙色素, 诱变

Abstract:

Microwave irradiation with water-cooling device was used for the mutagenesis of Monascus purpureus. The optimal microwave power, irradiation duration and dosage for the mutagenesis of Monascus purpureus were explored by evaluating the lethal and mutation regularity of Monascus purpureus. Results indicated that the optimal mutation conditions were microwave power of 500 W and irradiation duration of 80 s. Under the optimal microwave irradiation conditions, a mutated strain W5S8 with the highest productivity of orange pigments was achieved and the color value of orange pigments from strain W5S8 was 16.38 U/mL, which was increased by 59.62% compared with the parent strain. Its productivity for orange pigments remained stable after subculture for 5 generations or storage.

Key words: Monascus purpureus, microwave irradiation, orange pigment, mutation

中图分类号:

TS202.3

徐伟，范志诚，刘艳华. 微波辐照诱变选育高产橙色素红曲霉菌[J]. 食品科学, 2010, 31(23): 224-227.

XU Wei，FAN Zhi-cheng，LIU Yan-hua. Mutation Breeding of Monascus purpureus with High Productivity of Orange Pigments by Microwave Irradiation[J]. FOOD SCIENCE, 2010, 31(23): 224-227.

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微波辐照诱变选育高产橙色素红曲霉菌

Mutation Breeding of Monascus purpureus with High Productivity of Orange Pigments by Microwave Irradiation

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