食品科学 ›› 2010, Vol. 31 ›› Issue (23 ): 383-385.doi: 10.7506/spkx1002-6630-201023085

• 营养卫生 • 上一篇    下一篇

苦瓜皂苷对成骨细胞增殖和ALP 的影响

杨志刚,沈宗根,张燕萍   

  1. 常熟理工学院生物与食品工程学院
  • 收稿日期:2010-11-23 出版日期:2010-12-15 发布日期:2010-12-29
  • 通讯作者: 杨志刚 E-mail:zgy@cslg.cn
  • 基金资助:

    常熟理工学院青年教师科研启动基金项目(QZ0811)

Effect of Momordica charantia L. Saponins on Osteoblast Proliferation and Alkaline Phosphatase Expression

YANG Zhi-gang,SHEN Zong-gen,ZHANG Yan-ping   

  1. (College of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China)
  • Received:2010-11-23 Online:2010-12-15 Published:2010-12-29
  • Contact: YANG Zhi-gang E-mail:zgy@cslg.cn

摘要:

研究苦瓜皂苷对体外培养的新生SD 乳鼠成骨细胞增殖和ALP 的影响。以1 × 10-8~1 × 10-4mol/L 浓度梯度的苦瓜皂苷的MEM 含药培养液对新生 SD 乳鼠24h 内颅盖骨进行体外细胞培养,分别用 MTT 法和 PNPP 法检测成骨细胞的增殖和碱性磷酸酶(alkaline phosphatase,ALP)活性。结果表明:苦瓜皂苷具有时间和剂量依赖性地促进成骨细胞增殖和提高成骨细胞 ALP 活性。

关键词: 苦瓜皂苷, 成骨细胞, ALP

Abstract:

The present study was aimed at investigating the effect of Momordica charantia L. saponins on the proliferation and alkaline phosphatase (ALP) expression of in vitro cultivated osteoblasts of new born neonatal SD rats. The in vitro cultivation of new born SD rat (aged within 24 h) osteoblast at gradient concentrations of Momordica charantia L. saponins in MEM medium was performed, and MTT and PNPP assays were used to measure the proliferation and ALP activity of osteoblasts, respectively. Our findings suggest that Momordica charantia L. saponins promote the proliferation and ALP activity of osteoblasts in timeand dose-dependent manners.

Key words: Momordica charantia L. saponins, osteoblast, ALP

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