酒花黄酮提取工艺和含量测定

doi:10.7506/spkx1002-6630-201106004

食品科学 ›› 2011, Vol. 32 ›› Issue (6 ): 16-19.doi: 10.7506/spkx1002-6630-201106004

酒花黄酮提取工艺和含量测定

李敏¹，杨建华²，李渊¹，胡君萍^1,*

1.新疆医科大学药学院 2.新疆医科大学附属肿瘤医院

收稿日期:2010-04-19 修回日期:2011-01-18 出版日期:2011-03-25 发布日期:2011-03-03
通讯作者: 胡君萍 E-mail:hjp-yft@163.com
基金资助:
新疆维吾尔自治区高校科研计划重点项目(XJEDU2009I24)

Optimization of Extraction Process and Content Determination of Total Flavonoids in Humulus lupulus L.

LI Min1，YANG Jian-hua2，LI Yuan1，HU Jun-ping1,*

1. College of Pharmacy, Xinjiang Medical University, Urumqi 830011, China；
2. Affiliated Tumor Hospital, Xinjiang Medical University, Urumqi 830011, China

Received:2010-04-19 Revised:2011-01-18 Online:2011-03-25 Published:2011-03-03
Contact: HU Jun-ping1 E-mail:hjp-yft@163.com

摘要/Abstract

摘要： 建立酒花黄酮的提取方法和含量测定方法。采用NaNO2-Al(NO3)3-NaOH显色法，以芦丁为对照品，通过单因素试验和L9(34)正交设计确定酒花黄酮的提取方法，并测定和比较酒花、酒糟和啤酒中黄酮的含量。结果表明：酒花黄酮提取方法为样品加入50倍的甲醇，超声提取2次，每次1h，酒花黄酮含量为69.98mg/g，酒糟和啤酒中黄酮的含量较低。对照品芦丁在10.2～40.8μg/mL质量浓度范围内与吸光度呈良好线性关系，平均回收率99.33%，RSD=3.60%。该提取方法合理，含量测定方法简便、快速、准确，可用于酒花黄酮提取物制备及其质量控制。

关键词: 酒花, 黄酮, 提取工艺, 含量测定

Abstract: Ultrasonic extraction conditions for total flavonoids from Humulus lupulus L. were optimized by single factor experiments and orthogonal design with the aim of establishing a sample preparation method for determination of the content of total flavonoids in Humulus lupulus L. by NaNO2-Al(NO3)3-NaOH colorimetric method using rutin as the reference. The optimal extraction process parameters were material-to-liquid ratio of 1:50, extraction duration of 1 h and repeated extraction number of 2. The content of total flavonoids from Humulus lupulus L. was 69.98 mg/g under the optimal extraction conditions, which was much higher than that of residues and beer. The linear range was between 10.2μg/mL and 40.8μg/mL with a correlation coefficient of 0.9985. The average recovery rate was 99.33% with a relative standard deviation of 3.60%. The extraction and determination methods were simple and feasible, which is suitable for the preparation and quality control of flavonoids extracted from Humulus lupulus L.

Key words: Humulus lupulus L., total flavonoids, extraction, determination

中图分类号:

O623.54

李敏1，杨建华2，李渊1，胡君萍1,*. 酒花黄酮提取工艺和含量测定[J]. 食品科学, 2011, 32(6 ): 16-19.

LI Min1，YANG Jian-hua2，LI Yuan1，HU Jun-ping1,*. Optimization of Extraction Process and Content Determination of Total Flavonoids in Humulus lupulus L.[J]. FOOD SCIENCE, 2011, 32(6 ): 16-19.

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酒花黄酮提取工艺和含量测定

Optimization of Extraction Process and Content Determination of Total Flavonoids in Humulus lupulus L.

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