食品科学 ›› 2011, Vol. 32 ›› Issue (22): 11-15.doi: 10.7506/spkx1002-6630-201122003

• 工艺技术 • 上一篇    下一篇

响应面法优化玉米抗性淀粉制备工艺

张焕新1,2,于 博1,金征宇1,*   

  1. 1.江南大学食品学院,食品科学与技术国家重点实验室 2.江苏畜牧兽医职业技术学院食品科技学院
  • 出版日期:2011-11-25 发布日期:2011-11-11
  • 基金资助:
    江苏省自然科学基金创新学者攀登项目(BK2008003);江苏畜牧兽医职业技术学院科技项目(YB201004); 江苏省“青蓝工程”资助项目

Response Surface Methodology for Optimization of Maize Resistant Starch Preparation with Pullulanase

ZHANG Huan-xin1,2,YU Bo1,JIN Zheng-yu1,*   

  1. (1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China;2. School of Food Science and Technology, Jiangsu Animal Husbandry and Veterinary College, Taizhou 225300, China)
  • Online:2011-11-25 Published:2011-11-11

摘要: 研究普鲁兰酶法制备玉米抗性淀粉的工艺。在单因素试验基础上,采用响应曲面法研究pH值、反应温度、反应时间和加酶量对抗性淀粉得率的影响,优化玉米抗性淀粉制备工艺,建立各因素与抗性淀粉得率关系的数学回归模型。确定最佳的制备工艺条件为普鲁兰酶加酶量12.8ASPU/g、反应时间32h、反应温度46.2℃、pH5.0。在该制备条件下,抗性淀粉得率为46.2%。

关键词: 抗性淀粉, 普鲁兰酶, 玉米淀粉, 响应面

Abstract: In this study, resistant starch was prepared from maize starch with pullulanase. Based on one-factor-at-a-time experiments, the main parameters of pullulanase hydrolysis were investigated by response surface methodology (RSM), including reaction temperature, pH value, reaction time, and pullulanase amount, and a mathematical model describing resistant starch yield was developed. The optimum preparation conditions were achieved as follows: pullulanase amount 12.8 ASPU/g, reaction temperature 46.2 ℃, reaction time 32 h, pH 5.0. Under these conditions, the yield of the maize resistant starch is 46.2%.

Key words: resistant starch, pullulanase, maize starch, response surface methodology (RSM)

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