食品科学 ›› 2011, Vol. 32 ›› Issue (22): 46-50.doi: 10.7506/spkx1002-6630-201122010

• 工艺技术 • 上一篇    下一篇

响应面法优化樱桃籽清除DPPH自由基物质的提取工艺

姚东瑞1,2,郭 雷1,2,王淑军2,许福泉1   

  1. 1.淮海工学院 江苏省海洋生物技术重点实验室 2.淮海工学院海洋学院
  • 出版日期:2011-11-25 发布日期:2011-11-11
  • 基金资助:
    江苏省自然科学基金项目(BK2009631)

Response Surface Optimization of Extraction Process for DPPH Free Radical Scavenging Components from Cherry Seed

YAO Dong-rui1,2,GUO Lei1,2,WANG Shu-jun2,XU Fu-quan1   

  1. (1. Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China ; 2. Marine College, Huaihai Institute of Technology, Lianyungang 222005, China)
  • Online:2011-11-25 Published:2011-11-11

摘要: 优化樱桃籽抗氧化物质的提取工艺。以DPPH 自由基清除法检测提取物的抗氧化能力,考察3个变量(乙醇体积分数、提取温度和提取时间)对樱桃籽清除DPPH自由基的影响,并通过响应面分析法确定樱桃籽抗氧化物质提取的最佳工艺条件。结果表明:最佳工艺条件为液料比20:1(mL/g)、乙醇溶液体积分数33%、提取温度60℃、提取时间31min。在最佳提取条件下,DPPH 自由基实际清除率达到92.58%,与理论预测值非常接近,说明采用响应面法优化樱桃籽抗氧化物质的提取工艺可行。

关键词: 樱桃籽, 抗氧化物质, DPPH自由基清除活性, 响应面法

Abstract: In order to optimize the extraction of antioxidant components from cherry seed, an investigation into the effects of three operating parameters including ethanol concentration, temperature and extraction time on DPPH free radical scavenging activity of extracts was studied by means of response surface methodology (RSM). It was found that the optimal process conditions were liquid/material ratio 20:1 (mL/g), ethanol concentration 33% (V/V), extraction temperature 60 ℃, and extraction time 31 min. Under these extraction conditions, the DPPH free radical scavenging activity of the obtained extract from cherry seed was up to 92.58%, which was very close to the predicted value. Accordingly, the optimal process established in this study has good feasibility.

Key words: cherry seed, antioxidant components, DPPH free radical scavenging activity, response surface methodology

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