食品科学 ›› 2011, Vol. 32 ›› Issue (22): 128-134.doi: 10.7506/spkx1002-6630-201122027

• 工艺技术 • 上一篇    下一篇

响应面法优化葡萄皮渣中可溶性膳食纤维的酸法提取工艺

杜 彬1,李凤英2,范长军2,朱凤妹2,*   

  1. 1.河北科技师范学院分析测试中心 2.河北科技师范学院食品科技学院
  • 出版日期:2011-11-25 发布日期:2011-11-11
  • 基金资助:
    河北省高等学校科学技术研究青年基金项目(2010247);河北省科学技术研究与发展计划项目(09221009); 河北科技师范学院博士基金项目(2009YB005)

Response Surface Methodology for Optimization of Extraction Process for Soluble Dietary Fiber from Grape Pomace with Hydrochloric Acid

DU Bin1,LI Feng-ying2,FAN Chang-jun2,ZHU Feng-mei2,*   

  1. (1. Analysis and Testing Center, Hebei Normal University of Science and Technology, Changli 066600, China; 2. College of Food Science and Technology, Hebei Normal University of Science and Technology, Changli 066600, China)
  • Online:2011-11-25 Published:2011-11-11

摘要: 目的:利用响应面法对葡萄皮渣中可溶性膳食纤维的酸法提取工艺进行优化。方法:在单因素试验基础上选取试验因素与水平,根据Box-Behnken试验设计原理采用四因素三水平的响应面分析法,依据回归分析确定各工艺条件的影响因素,以可溶性膳食纤维得率为响应值作响应面和等高线图。结果:在分析各个因素的显著性和交互作用后,得出葡萄皮渣可溶性膳食纤维提取的最佳工艺为盐酸的浓度0.40mol/L、提取温度75℃、提取时间90min、料液比1:12(g/mL),在此工艺条件下可溶性膳食纤维得率为47.56mg/g。结论:响应面回归方程与实验结果拟合性好,此模型合理可靠,可用于实际预测。

关键词: 葡萄皮渣, 可溶性膳食纤维, 酸法, 响应面法

Abstract: Objective: To optimize the extraction of soluble dietary fiber (SDF) from grape pomace with hydrochloric acid. Methods: One-factor-at-a-time experiments, mathematical modeling based on Box-Behnken experimental design and multiple regression analysis and response surface analysis were carried out to investigate the individual and interactive effects of various process conditions on extraction yield of SDF and their significance. Response surface and contour plots were drawn with extraction yield of SDF as a function. Results: The optimal conditions for SDF extraction were 0.40 mol/L hydrochloric acid as extraction solvent at a material-to-liquid ratio of 1:12 (g/mL) and 75 ℃ for an extraction duration of 90 min. Under the optimal extraction conditions, the yield of SDF was up to 47.56 mg/g, which was close to the model-predicted value. Conclusion: The developed regression equation can be well fitted with experimental results and is reasonable, reliable and applicable for actual prediction.

Key words: grape pomace, soluble dietary fiber, acid method, response surface methodology

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