白背三七总黄酮的超声提取工艺及α- 葡萄糖苷酶抑制活性

doi:10.7506/spkx1002-6630-201204027

食品科学 ›› 2012, Vol. 33 ›› Issue (4): 134-139.doi: 10.7506/spkx1002-6630-201204027

白背三七总黄酮的超声提取工艺及α- 葡萄糖苷酶抑制活性

刘旭，刘微微，曹学丽

北京工商大学食品学院，食品添加剂与配料北京高校工程研究中心

出版日期:2012-02-25 发布日期:2012-02-14

Ultrasound-Assisted Extraction of Total Flavonoids from Stems and Leaves of Gynura divaricata (L.) DC. and Its Inhibitory Activity on α-Glucosidase

LIU Xu，LIU Wei-wei，CAO Xue-li

Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China

Online:2012-02-25 Published:2012-02-14

摘要/Abstract

摘要： 在单因素试验基础上，对乙醇体积分数、料液比和提取次数3因素进行Box-Behnken设计及响应面分析，优化白背三七黄酮超声提取条件，将白背三七粗提物进行分级萃取，并对不同级分萃取物的黄酮含量及α-葡萄糖苷酶抑制活性进行了考察。结果表明超声辅助提取白背三七中黄酮化合物的优化工艺参数为乙醇体积分数55%、料液比1:35(g/mL)、提取次数4次，在此条件下总黄酮得率为3.071%(30.71mg/g)，与预测值(3.095%)基本相符；经高效液相色谱分析，粗提物中主要化合物均具有黄酮特征吸收。分级萃取物中以乙酸乙酯相中黄酮含量及α-葡萄糖苷酶抑制活性最高。

关键词: 白背三七, 黄酮, 超声提取, 响应面分析, α-葡萄糖苷酶抑制活性

Abstract: On the basis of single-factor experiments, three key process parameters including ethanol concentration, solid/liquid ratio and extraction number for the ultrasound-assisted extraction of total flavonoids from the stems and leaves of Gynura divaricata (L.) DC. were optimized by Box-Behnken experimental design coupled with response surface methodology. The inhibitory effects of crude total flavonoid extract and its different solvent fractions on α-glucosidase were investigated. The results showed that the optimal ultrasound-assisted extraction conditions were 55% ethanol as extraction solvent at a material/liquid ratio of 1:35 (g/mL) for 4 repeated extractions. Under these conditions, the actual extraction yield of total flavonoids was 3.071%, which was basically consistent with the predicted value, 3.095%. HPLC analysis revealed that the major components in crude extract had the absorption characteristics of flavonoids.

Key words: Gynura divaricata (L.) DC., flavonoids, ultrasound-assisted extraction, response surface methodology, inhibitory activity on α-glucosidase

中图分类号:

R284.2

刘旭，刘微微，曹学丽. 白背三七总黄酮的超声提取工艺及α- 葡萄糖苷酶抑制活性[J]. 食品科学, 2012, 33(4): 134-139.

LIU Xu，LIU Wei-wei，CAO Xue-li. Ultrasound-Assisted Extraction of Total Flavonoids from Stems and Leaves of Gynura divaricata (L.) DC. and Its Inhibitory Activity on α-Glucosidase[J]. FOOD SCIENCE, 2012, 33(4): 134-139.

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白背三七总黄酮的超声提取工艺及α- 葡萄糖苷酶抑制活性

Ultrasound-Assisted Extraction of Total Flavonoids from Stems and Leaves of Gynura divaricata (L.) DC. and Its Inhibitory Activity on α-Glucosidase

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