食品科学 ›› 2012, Vol. 33 ›› Issue (5): 183-187.doi: 10.7506/spkx1002-6630-201205039

• 生物工程 • 上一篇    下一篇

半干面腐败菌的分离与鉴定

李 洁,孙 姝,朱科学,彭 伟,周惠明   

  1. 江南大学食品学院
  • 出版日期:2012-03-15 发布日期:2012-03-03

Isolation and Identification of Spoilage Microorganisms of Semi-dry Noodles

LI Jie,SUN Shu,ZHU Ke-xue,PENG Wei,ZHOU Hui-ming   

  1. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
  • Online:2012-03-15 Published:2012-03-03

摘要: 目的:探究引起半干面腐败变质的主要微生物类群。方法:采用形态学鉴定和生理生化鉴定的手段,对导致半干面腐败的主要微生物进行分离和鉴定。结果:半干面中以芽孢杆菌属(Bacillus)和葡萄球菌属(Staphylococcus)为优势腐败菌群;经分离鉴定的优势细菌有枯草芽孢杆菌(Bacillius  subtills)、地衣芽孢杆菌(B. licheniformis)、表皮葡萄球菌(Staphylococcus epidermidis)和金黄色葡萄球菌(S. aureus);分离鉴定的霉菌主要有白曲霉(Aspergillus candidus)、杂色曲霉(A. versicolor)和圆弧青霉(Penicillum cyclopium)。芽孢杆菌是半干面贮藏前期最主要的细菌,但随着贮藏时间的延长,葡萄球菌大量生长繁殖,成为半干面中的优势细菌。

关键词: 半干面, 腐败菌, 分离, 鉴定

Abstract: Objective: To explore the major spoilage microorganisms in semi-dry noodles and to provide theoretical basis of preservation. Methods: The major microorganisms in semi-dry noodles were isolated and identified by means of morphological and biochemical tests. Results: The dominant spoilage microorganisms were Bacillus and Staphylococcus. The isolated bacteria strains were Bacillus subtills, B. licheniformis, Staphylococcus epidermidis and S. aureus. The isolated mould strains belonged to the species of Aspergillus candidus, A. versicolor and Penicillum cyclopium. During the first 2 days, species of B. subtills and B. licheniformis were dominant. After 2 days of storage, S. epidermidis became the dominant bacteria in the semi-dry noodles and S. aureus was also detected.

Key words: semi-dry noodles, spoilage microorganism, isolation, identification

中图分类号: