食品科学 ›› 2012, Vol. 33 ›› Issue (6): 83-86.doi: 10.7506/spkx1002-6630-201206018

• 工艺技术 • 上一篇    下一篇

大孔树脂分离纯化粘性红圆酵母产β-胡萝卜素

刘卉琳1,刘 绍1,2,周月华3,贾 涛4,谢达平1   

  1. 1.湖南农业大学生物安全科学技术学院 2.湖南农业大学食品科技学院 3.安捷伦科技(上海)有限公司 4.江苏省生产力促进中心 江苏省理化测试中心
  • 出版日期:2012-03-25 发布日期:2012-03-03

Isolation and Purification of β-Carotene from Rhodotorula mucilaginosa by Macroporous Resin Adsorption

LIU Hui-lin1,LIU Shao1,2,ZHOU Yue-hua3,JIA Tao4,XIE Da-ping1   

  1. (1. College of Biosafety Science and Technology, Hunan Agricultural University, Changsha 410128, China; 2. College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China; 3. Agilent Technologies (Shanghai) Co. Ltd., Shanghai 200131, China; 4. Physics and Chemistry Test Center of Jiangsu Province, Jiangsu Productivity Promotion Centre, Nanjing 210042, China)
  • Online:2012-03-25 Published:2012-03-03

摘要: 利用大孔树脂对粘性红圆酵母RM-1产β-胡萝卜素进行分离纯化,得到最佳的吸附和解吸条件。结果表明,最佳吸附树脂为X-5树脂,最佳洗脱剂为乙醚,最佳分离纯化工艺参数为上样质量浓度111.82μg/mL、吸附流速1mL/min、洗脱流速0.5mL/min。经纯化,β-胡萝卜素纯度达到33.29%,与未纯化相比,提高了6.87倍。

关键词: 粘性红圆酵母, β-胡萝卜素, 大孔吸附树脂, 分离纯化

Abstract: The β-carotene produced by Rhodotorula mucilaginosa RM-1 was purified by macroporous adsorption resin and its optimal adsorption and desorption conditions were achieved in this paper. The results showed that X-5 resin was the optimal adsorbent and ether was the optimal eluent. The optimum static adsorption and desoprtion conditions were sample concentration of 111.82μg/mL, flow rate for adsorption of 1 mL/min, and flow rate for desorption of 0.5 mL/min. Under these optimal conditions, the purity of β-carotene was up to33.29% and the purification fold was 6.87。

Key words: Rhodotorula mucilaginosa, β-carotene, macroporous resin, isolation and purification

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