大孔树脂纯化蓝莓叶多酚及其组成分析

doi:10.7506/spkx1002-6630-201310019

食品科学 ›› 2013, Vol. 34 ›› Issue (10): 86-91.doi: 10.7506/spkx1002-6630-201310019

大孔树脂纯化蓝莓叶多酚及其组成分析

^{冯进，李敏，曾晓雄，李春阳}

1.江苏省农业科学院农产品加工研究所，江苏南京 210014；2.南京农业大学食品科技学院，江苏南京 210095；
3.南京财经大学食品科学与工程学院，江苏省粮油品质控制及深加工技术重点实验室，江苏南京 210003

收稿日期:2012-03-16 修回日期:2013-04-08 出版日期:2013-05-25 发布日期:2013-05-07
通讯作者: 李春阳 E-mail:yz3737@yahoo.com.cn
基金资助:
2011年南京市科技发展计划国际联合研发项目

Macroporous Resin Purification and Composition Analysis of Polyphenols from Blueberry Leaves

FENG Jin，LI Min，ZENG Xiao-xiong，LI Chun-yang

1. Institute of Farm Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China；
2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China；
3. Key Laboratory of Grain and Oil Quality Control and Deep-Utilizing Technology of Jiangsu Province, College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210003, China

Received:2012-03-16 Revised:2013-04-08 Online:2013-05-25 Published:2013-05-07

摘要/Abstract

摘要：

比较8种大孔树脂对蓝莓叶多酚的静态吸附与解吸效果，HPD400树脂吸附量为52.95mg/g，解吸率为95.96%，并且达到吸附平衡与解吸平衡的时间较短，最适合蓝莓叶多酚的纯化。当上样质量浓度3mg/mL、pH2、上样流速2mL/min、上样体积140mL时，树脂带到动态吸附饱和；用80mL 40%的乙醇，以1mL/min的流速进行洗脱，可以洗脱完全，解吸率为90.50%。经过HPD400树脂的纯化，蓝莓叶多酚的纯度由原来的38.75%提高到69.38%。HPLC-DAD-MS的分析结果显示，蓝莓叶多酚含有丰富的咖啡酰奎宁酸与槲皮素甙。

关键词: 蓝莓叶多酚, 大孔树脂, 纯化, HPLC-DAD-MS

Abstract:

Adsorption and desorption of 8 types of macroporous resins towards polyphenols from blueberry leaves were
investigated. The adsorption capacity and desorption rate of HPD400 resin was 52.95 mg/g and 95.96% respectively, and the
time to reach equilibrium between adsorption and desorption was short, so it was the most suitable resin to purify blueberry
leaves polyphenols. When 140 mL of 3 mg/mL samples with pH 2 was flowed through the HPD400 resin at a speed of 1
mL/min, the adsorption reached dynamic equilibrium. The adsorbed polyphenols could be completely eluted with 80 mL of
40% ethanol at 1 mL/min, resulting in a desorption rate of 90.50%. After being purified with HPD400 resin, the purity of
polyphenols was increased from 38.75% to 69.38%. HPLC-DAD-MS analysis showed that the blueberry leaf polyphenols
were rich in caffeoylquinic acids and quercetin glycosides.

Key words: blueberry leaves polyphenols, macroporous resin, purification, HPLC-DAD-MS

中图分类号:

TS201.1

冯进，李敏，曾晓雄，李春阳. 大孔树脂纯化蓝莓叶多酚及其组成分析[J]. 食品科学, 2013, 34(10): 86-91.

FENG Jin，LI Min，ZENG Xiao-xiong，LI Chun-yang. Macroporous Resin Purification and Composition Analysis of Polyphenols from Blueberry Leaves[J]. FOOD SCIENCE, 2013, 34(10): 86-91.

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大孔树脂纯化蓝莓叶多酚及其组成分析

Macroporous Resin Purification and Composition Analysis of Polyphenols from Blueberry Leaves

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