雌、雄皱纹盘鲍内脏脂肪酸及磷脂组成的比较分析

doi:10.7506/spkx1002-6630-201310040

食品科学 ›› 2013, Vol. 34 ›› Issue (10): 184-186.doi: 10.7506/spkx1002-6630-201310040

雌、雄皱纹盘鲍内脏脂肪酸及磷脂组成的比较分析

^{刘艳青，李兆杰*，李国云，刘小芳，薛长湖}

中国海洋大学食品科学与工程学院，山东青岛 266003

收稿日期:2012-02-24 修回日期:2013-04-09 出版日期:2013-05-25 发布日期:2013-05-07
通讯作者: 刘艳青 E-mail:lyqtheone@163.com
基金资助:
国际科技合作项目

Comparative Analysis of Fatty Acid and Phospholipids in Viscera of Male and Female Haliotis discus hannai Ino

LIU Yan-qing，LI Zhao-jie*，LI Guo-yun，LIU Xiao-fang，XUE Chang-hu

College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China

Received:2012-02-24 Revised:2013-04-09 Online:2013-05-25 Published:2013-05-07
Contact: Liu YanQing E-mail:lyqtheone@163.com

摘要/Abstract

摘要：

目的：对雌、雄皱纹盘鲍内脏脂质进行提取及测定，主要对其脂肪酸和磷脂组成进行分析比较。方法：Floch法提取雌、雄鲍鱼内脏脂质，测定脂质基本指标，游离脂肪酸、甘油三酯、磷脂、胆固醇含量；气相色谱法测定雌、雄鲍鱼内脏脂肪酸组成，并对其比较分析；固相微萃取法分别分离提取雌、雄鲍鱼内脏磷脂，高效液相色谱-蒸发光散射法测定雌、雄鲍鱼内脏磷脂组成并比较分析。结果：雌、雄皱纹盘鲍内脏脂质有较大差异，雌性鲍鱼内脏不饱和脂肪酸含量高于雄性；雌、雄鲍鱼内脏磷脂成分较复杂，但主要的磷脂组分为磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰丝氨酸。结论：鲍鱼内脏中含有丰富的磷脂及多不饱脂肪酸，具有较好的食用和药用价值，应得到充分利用。

关键词: 雌雄鲍鱼内脏, 脂肪酸, 磷脂, 气相色谱, 高效液相色谱-蒸发光散射

Abstract:

Objective: To describe comparative analysis of visceral fatty acids and phospholipids in male and female Haliotis
discus hannai Ino. Methods: Visceral total lipids were extracted using Folch method and determined for the contents of free
fatty acids, triglycerides, phospholipids and cholesterol. The fatty acid composition was analyzed by GC after derivatization
with 10% H2SO4-CH3OH solution. Phospholipids were extracted using SPE method, and then analyzed by HPLC-ELSD.
Results: The visceral content of unsaturated fatty acids in female Haliotis discus hannai Ino was higher than in male Haliotis
discus hannai Ino. The composition of visceral phospholipids in male and female Haliotis discus hannai Ino, though
complicated, mainly consisted of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylserine (PS).
Conclusion: The viscera of both female and male Haliotis discus hannai Ino are rich in phospholipids and polysaturated fatty
acids and have high medicinal and edible values.

Key words: viscera of Haliotis discus hannai Ino, fatty acids, phospholipids, gas chromatography (GC), high performance liquid chromatography-evaportive light scattering detector (HPLC-ELSD)

中图分类号:

刘艳青，李兆杰*，李国云，刘小芳，薛长湖. 雌、雄皱纹盘鲍内脏脂肪酸及磷脂组成的比较分析[J]. 食品科学, 2013, 34(10): 184-186.

LIU Yan-qing，LI Zhao-jie*，LI Guo-yun，LIU Xiao-fang，XUE Chang-hu. Comparative Analysis of Fatty Acid and Phospholipids in Viscera of Male and Female Haliotis discus hannai Ino[J]. FOOD SCIENCE, 2013, 34(10): 184-186.

参考文献

[1] 李太武, 苏秀榕, 丁明进. 皱纹盘鲍中几种营养成分的测定[J]. 中国海洋药物杂志, 1995, 1 : 47-48.

[2] 王兵,蒋建敏,许东晖,等. 鲍鱼多糖对人鼻咽癌裸鼠抗癌作用的研究[J ] . 中草药,2000 ,31 (8) :597.

[3] 王兵,许东晖,许实波,等. 鲍鱼多糖对环磷酰胺的增效减毒作用[J ] . 中药材,1999 ,22 (4) :198.

[4] 许东晖, 王兵, 许实波, 等. 鲍鱼多糖对荷瘤小鼠腹腔巨噬细胞活性及迟发型超敏反应的作用[J]. 中药材, 1999, 22(2): 88-89.

[5] 许东晖, 许实波, 王兵, 等. 皱纹盘鲍多糖抗肿瘤药理作用研究[J]. 热带海洋学报, 1999, 18(4):86-90.

[6] 朱莉莉, 孙黎明, 李冬梅, 等. 鲍鱼内脏蛋白多糖体内对H22肝癌的抑制作用[J]. 营养学报, 2009, 31(5): 478-482.

[7] 楼乔明，王玉明,等. 皱纹盘鲍脂肪酸及脂肪醛二甲基缩醛的气相色谱/质谱分析[J]. 中国海洋大学学报，2010，41( 6) : 041~ 044.

[8] 王竹清,李八方,李平林等. 皱纹盘鲍性腺脂质成分分析[J]. 中国海洋药物，2011,30(3):37-42.

[9] García-Regueiro J A，Gibert J，Díaz I. Determination of neutral lip ids from subcutaneous fat of cured ham by capillary gas chromatography and liquid chromatography. Journal of Chromatography A, 1994, 667: 225 - 233.

[10] L.M. Rodríguez-Alcalá, J. Fontecha. Major lipid classes separation of buttermilk, and cows, goats and ewes milk by high performance liquid chromatography with an evaporative light scattering detector focused on the phospholipid fraction. Journal of Chromatography A, 1217 (2010) 3063–3066.

[11] Naito S．Efect of dietary diaeylglyeerols on lipids metobilism in human：1． The suspressive efect of dietary diacylglyeerols on the increase of the serum triglyeerides[Z]．1rhe 16th International Congress ofNutrition．．1997．341.

[12] [16]Hashioka S, Han YH，Fujii S, Kato T，Monji A.Utsumi H , Sawada M , Nakanishi H,.Kanba S. Ph0sphatidylserine and phosphatidylcholine—containing liposomes inhibit amyloid p and interferon一induced microglial activation[J].Free Radical Biology and Medicine, 2007,.42(7):945-954．

[13] Engel RR，Satzger W, Gtinther W, Kathmann N, Bove D, Gerke S，Mtinch U，Hippius H．Double—blind cross—over study of ph0sphatidylserine vs. placebo in patients with early dementia of the Alzheimer type[J]. European Neuropsychopharmacology, 1992, 2(2):149～155.

[14] Alves CS, Andreatini R, Cunha CD, Tufik S, Vital MA, PhosDhatidvlserine reverses reserpine—induced amnesia[J]. European Journal of Pharmacology,2000, 404(1,2):161～167.

[15] Claro FY, Patti CL, Abflio VC, Filho RF, Silva RH. Bovine brain phosphatidvlserine attenuates scopolamine induced amnesia in mice[J]．Progress in Neuro—Psychopharmacology and

Biological Psychiatry, 2006, 30(5):881～886.

雌、雄皱纹盘鲍内脏脂肪酸及磷脂组成的比较分析

Comparative Analysis of Fatty Acid and Phospholipids in Viscera of Male and Female Haliotis discus hannai Ino

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	刘玉兰，舒垚，孙国昊，马宇翔，姜元荣，陈宁. 花生品种对花生酱风味及综合品质的影响[J]. 食品科学, 2021, 42(9): 15-21.
[2]	王勇，宋歌，庞邵杰，綦文涛. 应用Illumina NovaSeq测序技术比较3 种杂粮对大鼠肠道菌群的影响[J]. 食品科学, 2021, 42(9): 100-106.
[3]	牙甫礼，张春梅，陈彬林，谷仕艳，贾小娥. 辅酶Q10经蛋白激酶A/胞浆型磷脂酶A2信号通路抑制血小板血栓素A2的生成[J]. 食品科学, 2021, 42(9): 130-136.
[4]	王淳纯，覃小丽，阚建全，刘雄，索化夷，钟金锋. 量子化学计算法比较不同脂肪酸分子的反应活性位点[J]. 食品科学, 2021, 42(8): 74-80.
[5]	单启梅，赵晓策，罗瑞明，尤丽琴. 滩羊肌肉在煮制过程中可挥发性化合物的变化[J]. 食品科学, 2021, 42(8): 165-171.
[6]	王富云，王成财，王哲铭，江虹锐，闵斗勇，刘小玲，李树波. 虾青素微球对酸奶品质与风味的影响[J]. 食品科学, 2021, 42(8): 172-178.
[7]	曹荣，胡梦月，谭志军，王联珠，刘淇. 基于电子舌和气相色谱-离子迁移谱分析坛紫菜与条斑紫菜的风味特征[J]. 食品科学, 2021, 42(8): 186-191.
[8]	刘盼盼，郑鹏程，龚自明，常泽睿，苏方俊，黄波，冯琳，高士伟，郑琳. 橘红茶香气特征及风味成分分析[J]. 食品科学, 2021, 42(8): 198-205.
[9]	张舒，王长远，冯玉超，盛亚男，富天昕，张艺玮，姜颖俊，于淼，张丽媛. 气相色谱-质谱联用代谢组学技术分析不同产地稻米代谢物[J]. 食品科学, 2021, 42(8): 206-213.
[10]	甘玉飞，薛正莲，周杰，王芳，王洲，刘艳. 黏质沙雷菌磷脂酶A1辅助蛋白S对表达宿主菌大肠杆菌抑制作用机制[J]. 食品科学, 2021, 42(7): 45-51.
[11]	吴聪，葛科立，路宗博，郑征，张金玉，薛美兰，葛银林. n-3多不饱和脂肪酸对小鼠血液外泌体中miRNA的调节和抑制肥胖作用[J]. 食品科学, 2021, 42(7): 176-184.
[12]	杨蒙，薛正莲，甘玉飞，周杰，王洲，刘艳. 辅助蛋白基因的剪接对磷脂酶A1酶活的影响[J]. 食品科学, 2021, 42(6): 104-110.
[13]	陈丽花，任丽霞，李东娜，马霞. 甜酒曲中优质酵母菌的分离鉴定及其产香特性分析[J]. 食品科学, 2021, 42(6): 142-149.
[14]	彭潇，邹文静，邵清清，孙中贯，张立华，李贺贺，王雪山. 石榴酒发酵过程中真菌种群演替及风味物质代谢规律解析[J]. 食品科学, 2021, 42(6): 157-163.
[15]	裴紫薇，杨杰，张磊，周爽，赵云峰，陈达炜，苗虹，方从容. 载气类型对气相色谱法分离顺-反脂肪酸异构体的影响[J]. 食品科学, 2021, 42(6): 200-205.