食品科学

• 生物工程 • 上一篇    下一篇

偏高温大曲发酵过程中可培养细菌群落研究

周瑞平1,王 涛2,陈云宗1,江东材1,唐代云1,刘 超1   

  1. 1.四川省宜宾市叙府酒业股份有限公司技术中心,四川 宜宾 644000;
    2.宜宾学院 发酵资源与应用四川省高校重点实验室,四川 宜宾 644000
  • 出版日期:2013-07-15 发布日期:2013-06-28
  • 通讯作者: 周瑞平
  • 基金资助:

    四川省科技厅科技支撑计划项目(2010SZ0229);四川省经信委重大产业创新专项(2011YB00275);
    宜宾市自主创新项目(200905207);宜宾市科技创新专项(2011ZGY009)

Culturable Bacterial Flora in the Fermentation of High-temperature Daqu

ZHOU Rui-ping1,WANG Tao2,CHEN Yun-zong1,JIANG Dong-cai1,TANG Dai-yun1,LIU Chao1   

  1. 1. Technical Center of Yibin Xufu Liquor Industry Co. Ltd., Yibin 644000, China;2. Key Laboratory of Fermentation Resource
    and Application of Institutes of Higher Learning in Sichuan Province, Yibin University, Yibin 644000, China
  • Online:2013-07-15 Published:2013-06-28
  • Contact: ZHOU Rui-ping1

摘要:

采用经典微生物学方法和16S rDNA基因系统发育分析的方法,对偏高温大曲发酵过程中的细菌类群及 其演替情况进行分析。结果显示:在偏高温大曲发酵过程中,细菌数量从拌料的1.0×107个/g快速上升到最大值 4.0×108个/g(第一次翻曲,4d),最后降低到1.5×107个/g;可培养的细菌类群从最开始的5属9种增加到9属26种后减 少至第一次翻曲时(4d)的7属23种,随后多样性持续下降,到收曲时仅检测到1属6种。同时发现在整个发酵过程中 Bacillus为绝对优势属,在整个发酵环节均存在。

关键词: 偏高温大曲, 细菌类群演替, 16S rDNA

Abstract:

Using classical microbiological methods and 16S rDNA gene phylogenetic analysis of the culturable bacterial
flora in the fermentation of high-temperature Daqu, we found that the number of bacteria at the first stirring (on the 4th d) was
rapidly increased to a maximum level of 4.0 × 108 CFU /g from the initial level of 1.0 × 107 CFU/g, and then was reduced
to 1.5 × 107 CFU/g finally. The cultivable bacterial populations were increased to 26 species in 9 genus from 9 species in
5 genus at the beginning, and then were reduced to 23 species in 7 genus at the first stirring (on the 4th d), and during the
following stages the bacterial diversity continued to reduce to 6 species in 1 genus finally. In addition, we also found Bacillus
existed throughout the fermentation process as the dominant genus.

Key words: high-temperature Daqu, culturable bacterial flora, 16S rDNA

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