食品科学
• 生物工程 • 上一篇 下一篇
赵育卉,王晓敏,王 惠,彭 洁,刘天行,辛志宏*
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ZHAO Yu-hui,WANG Xiao-min,WANG Hui,PENG Jie,LIU Tian-xing,XIN Zhi-hong*
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摘要:
对分离自江苏盐城大丰港盐碱地野生海芦笋的两株嗜盐内生真菌进行分离鉴定,提取基因组DNA后分别对18S rDNA和rDNA ITS1-5.8S-ITS4区域进行PCR扩增,基因测序并进行同源性分析,构建进化树并进行系统发育分析,结合形态学观察,将该两株菌分别鉴定为维管束茎点霉(Phoma tracheiphila)与镰刀菌属(Fusarium sp.)。
关键词: 嗜盐真菌, 形态学, 18S rDNA序列, rDNA ITS1-5.8S-ITS4序列, 系统发育分析
Abstract:
In this study, two strains of endophytic fungi were isolated and identified from Salicornia bigelovii collected from the beach of Dafeng harbor, Yancheng city, Jiangsu province in China. The 18S rDNA and rDNA ITS1-5.8S-ITS4 regions of these strains were amplified by polymerase chain reaction (PCR) after the extraction of mycelial genomic DNA and the amplification products were subjected to sequencing and homology analysis. A phylogenetic tree was established based on the sequences of 18S rDNA and internal transcribed spacer (ITS) region, combined with morphological observation. Therefore, both strains were identified as Phoma tracheiphila and Fusarium sp., respectively.
Key words: Salicornia bigelovii, morphology, 18S rDNA sequence, ITS1-5.8S-ITS4 sequence, phylogenetic analysis
中图分类号:
TS255.1
赵育卉,王晓敏,王 惠,彭 洁,刘天行,辛志宏*. 两株盐生海芦笋内生真菌的分离与鉴定[J]. 食品科学, doi: 10.7506/spkx1002-6630-201315030.
ZHAO Yu-hui,WANG Xiao-min,WANG Hui,PENG Jie,LIU Tian-xing,XIN Zhi-hong*. Isolation and Identification of Two Endophytic Fungus Strains from Salicornia bigelovii[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201315030.
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链接本文: https://www.spkx.net.cn/CN/10.7506/spkx1002-6630-201315030
https://www.spkx.net.cn/CN/Y2013/V34/I15/148