关键词: 富铬灵芝, 发酵培养基, 优化, Plackett-Burman设计, 中心组合设计, 响应面法

Abstract:

In the current study, the appropriate concentration range of CrCl3•6H2O in the fermentation medium for chromiumenriched
Ganoderma lucidum was established by one-factor-at-a-time design. CrCl3•6H2O, soybean cake powder and
corn meal were identified by Plackett-Burman (PB) design as main medium components that influence the total chromium
content of G. lucidum mycelia and the steepest ascent method was used to approach the neighborhood of the optimum. A
quadratic regression model with the total chromium content of G. lucidum mycelia as the dependent variable was established
by central composite design (CCD). The optimum fermentation medium composition was determined by response surface
analysis as 250.5 mg/L CrCl3•6H2O, 13.07 g/L soybean cake powder, 27.35 g/L corn meal, 18 g/L sucrose, 0.012 g/L VB1,
1.6 g/L NH4Cl, 0.8 g/L MgSO4•7H2O and 0.8 g/L KH2PO4. The measured value of total chromium content was 1525.92 μg/L
under the optimal conditions, which was well consistent with the predicted value of 1530.21 μg/L. Hence the prediction model
was available and reliable for the optimization of fermentation medium for chromium-enriched G. lucidum.

Key words: chromium-enriched Ganoderma lucidum, fermentation medium, optimization, Plackett-Burman (PB) design, center composite design, response surface methodology (RSM)